Evidence map›Paper›PMID 29449377›Full record

ArticleThe Journal of biological chemistry2018

Haploid embryonic stem cells can be enriched and maintained by simple filtration.

Chao Qu, Meng Yan, Suming Yang, Lingbo Wang, Qi Yin, Yuan Liu, Yeguang Chen, Jinsong Li

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Gigabase-scale deletion scanning of the human genome.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. The milestone of genetic screening: Mammalian haploid cells.Computational and structural biotechnology journal · 2020
    Review
  7. Mammalian haploid stem cells: establishment, engineering and applications.Cellular and molecular life sciences : CMLS · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Chao QuFrom the State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China and.
Meng YanFrom the State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China and.
Suming YangFrom the State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China and.
Lingbo WangFrom the State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China and.
Qi YinFrom the State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China and.
Yuan Liuthe State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Yeguang Chenthe State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Jinsong LiFrom the State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China and jsli@sibcb.ac.cn.
University of Chinese Academy of Sciences · CNCenter for Excellence in Molecular Cell Science · CNTsinghua University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mammalian haploid embryonic stem cells (haESCs) serve as a powerful tool for genetic analyses at both the cellular and organismal levels. However, spontaneous diploidization of haESCs limits their use in these analyses. Addition of small molecules to the culture medium to control the cell cycle can slow down diploidization, but cell-sorting methods such as FACS are still required to enrich haploid cells for long-term maintenance

Indexed as

AnimalsBlastocystCell Culture TechniquesCell LineCells, CulturedCell SizeCRISPR-Cas SystemsDiploidyEmbryonic Stem CellsFiltrationGene EditingGenetic TestingHaploidyMiceMouse Embryonic Stem CellsOocytescell biologycell sortingCRISPR/Casdiploidizationembryoembryonic stem cellFACSfiltrationhaploid embryonic stem cellshaploidy

Identifiers

PMID29449377
PMCPMC5892596
OpenAlexW2787093951

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.