ArticleMolecular & cellular proteomics : MCP2018
Defeating Major Contaminants in Fe
Article in Molecular & cellular proteomics : MCP, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 91 citations in OpenAlex.
- Article
- Article
- Advanced Phosphoproteomics Depth and Quantitative Performance Using an Integrated noFAIMS-FAIMS Approach.Rapid communications in mass spectrometry : RCM · 2026Article
- CAMK2D causes heart failure in mice with RBM20 cardiomyopathy.Nature cardiovascular research · 2026Article
- mTORC1 senses glutamine and other amino acids through GCN2.The EMBO journal · 2025Article
- Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.Molecular systems biology · 2025Article
- Uncovering protein glycosylation dynamics and heterogeneity using deep quantitative glycoprofiling (DQGlyco).Nature structural & molecular biology · 2025Article
- In-Depth Analysis of Tissue Phosphoproteomics with TiOMethods in molecular biology (Clifton, N.J.) · 2025Article
- [Progress in enrichment methods for proteinSe pu = Chinese journal of chromatography · 2024Review
- [Preparation of magnetic carbon nitride composite toward phosphopeptide enrichment].Se pu = Chinese journal of chromatography · 2024Article
- Review
- Dynamic Protein Phosphorylation inMicroorganisms · 2024Article
- A multidimensional atlas of human glioblastoma-like organoids reveals highly coordinated molecular networks and effective drugs.NPJ precision oncology · 2024Article
- Interferon regulates neural stem cell function at all ages by orchestrating mTOR and cell cycle.EMBO molecular medicine · 2023Article
- A Review of the Bacterial Phosphoproteomes of Beneficial Microbes.Microorganisms · 2023Review
- ASAP─Automated Sonication-Free Acid-Assisted Proteomes─from Cells and FFPE Tissues.Analytical chemistry · 2023Article
- Protein-Peptide Turnover Profiling reveals the order of PTM addition and removal during protein maturation.Nature communications · 2022Article
- Widespread Arginine Phosphorylation in Staphylococcus aureus.Molecular & cellular proteomics : MCP · 2022Article
- A Novel Spectral Annotation Strategy Streamlines Reporting of Mono-ADP-ribosylated Peptides Derived from Mouse Liver and Spleen in Response to IFN-γ.Molecular & cellular proteomics : MCP · 2022Article
- Clostridioides difficile Phosphoproteomics Shows an Expansion of Phosphorylated Proteins in Stationary Growth Phase.mSphere · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with immobilized metal ion affinity chromatography (IMAC)-based phosphopeptide enrichments. We address and largely solve this issue, developing a robust protocol implementing methanol/chloroform protein precipitation and enzymatic digestion using benzonase, which degrades all forms of DNA and RNA, before IMAC-column loading. This simple procedure resulted in a drastic increase of enrichment sensitivity, enabling the identification of around 17,000 unique phosphopeptides and 12,500 unambiguously localized phosphosites in human cell-lines from a single LC-MS/MS run, constituting a 50% increase when compared with the standard protocol. The improved protocol was also applied to bacterial samples, increasing the number of identified bacterial phosphopeptides even more strikingly, by a factor 10, when compared with the standard protocol. For
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.