ArticlePloS one2018
Strain-specific quantification of root colonization by plant growth promoting rhizobacteria Bacillus firmus I-1582 and Bacillus amyloliquefaciens QST713 in non-sterile soil and field conditions.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 122 citations in OpenAlex.
- Microbial Community Shifts and Plant Performance Improvements Driven by Bacillus firmus in Pampa Agroecosystems.Environmental microbiology reports · 2026Article
- Specific monitoring of viable plant growth-promoting Bacillus velezensis PH-023 in the rhizosphere of tomato.BMC microbiology · 2026Article
- Halotolerant Rhizobacteria from Phragmites Communis: A Controlled Proof-of-Concept for Crop Improvement in Degraded Sandy Soils.Microorganisms · 2026Article
- Engineering phosphoribosylpyrophosphate synthetase in Methylobacterium radiotolerans for lettuce growth and quality enhancement.Archives of microbiology · 2026Article
- Biocontrol Potential of Three Bacterial Strains, Isolated from Vermicompost, Against Pre-Emergence Damping-Off of Tomato Caused byMicroorganisms · 2026Article
- Development and Utilization of Quantitative Detection for Probiotics Bacillus velezensis B31 with Potential Protection Against Tomato Fusarium Wilt.Probiotics and antimicrobial proteins · 2026Article
- Synthetic Microbial Communities Enhance Pepper Growth and Root Morphology by Regulating Rhizosphere Microbial Communities.Microorganisms · 2025Article
- Monitoring the Persistence of Pseudomonas sivasensis Strain CF10PS3 in Cereal Fields.MicrobiologyOpen · 2024Article
- Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth.Nature communications · 2024Article
- Real-Time PCR (qtPCR) to Discover the Fate of Plant Growth-Promoting Rhizobacteria (PGPR) in Agricultural Soils.Microorganisms · 2024Article
- Rhizobiome Transplantation: A Novel Strategy beyond Single-Strain/Consortium Inoculation for Crop Improvement.Plants (Basel, Switzerland) · 2023Review
- Plant growth promotion and differential expression of defense genes in chilli pepper against Colletotrichum truncatum induced by Trichoderma asperellum and T. harzianum.BMC microbiology · 2023Article
- Harnessing NativeApplied and environmental microbiology · 2023Review
- Optimization of Molecular Methods for Detecting Duckweed-Associated Bacteria.Plants (Basel, Switzerland) · 2023Article
- The Role of Plant Growth-Promoting Microorganisms (PGPMs) and Their Feasibility in Hydroponics and Vertical Farming.Metabolites · 2023Review
- Evaluation of Selenium Nanoparticles in Inducing Disease Resistance against Spot Blotch Disease and Promoting Growth in Wheat under Biotic Stress.Plants (Basel, Switzerland) · 2023Article
- The Effects of Bacillus subtilis QST713 and β-mannanase on growth performance, intestinal barrier function, and the gut microbiota in weaned piglets.Journal of animal science · 2023Article
- Colonization and population dynamics of total, viable, and culturable cells of two biological control strains applied to apricot, peach, and grapevine crops.Frontiers in microbiology · 2023Article
- Review
- Pangenomes-identified singletons for designing specific primers to identify bacterial strains in a plant growth-promoting consortium.Molecular biology reports · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus amyloliquefaciens QST713 and B. firmus I-1582 are bacterial strains which are used as active ingredients of commercially-available soil application and seed treatment products Serenade® and VOTiVO®, respectively. These bacteria colonize plant roots promoting plant growth and offering protection against pathogens/pests. The objective of this study was to develop a qPCR protocol to quantitate the dynamics of root colonization by these two strains under field conditions. Primers and TaqMan® probes were designed based on genome comparisons of the two strains with publicly-available and unpublished bacterial genomes of the same species. An optimized qPCR protocol was developed to quantify bacterial colonization of corn roots after seed treatment. Treated corn seeds were planted in non-sterile soil in the greenhouse and grown for 28 days. Specific detection of bacteria was quantified weekly, and showed stable colonization between ~104-105 CFU/g during the experimental period for both bacteria, and the protocol detected as low as 103 CFU/g bacteria on roots. In a separate experiment, streptomycin-resistant QST713 and rifampicin-resistant I-1582 strains were used to compare dilution-plating on TSA with the newly developed qPCR method. Results also indicated that the presence of natural microflora and another inoculated strain does not affect root colonization of either one of these strains. The same qPCR protocol was used to quantitate root colonization by QST713 and I-1582 in two corn and two soybean varieties grown in the field. Both bacteria were quantitated up to two weeks after seeds were planted in the field and there were no significant differences in root colonization in either bacteria strain among varieties. Results presented here confirm that the developed qPCR protocol can be successfully used to understand dynamics of root colonization by these bacteria in plants growing in growth chamber, greenhouse and the field.
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