ArticlePloS one2018
Engineered resistance to Nosema bombycis by in vitro expression of a single-chain antibody in Sf9-III cells.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Towards a Rational Basis for the Selection of Probiotics to Improve Silkworm Health and Performance.Insects · 2025Review
- Microsporidian Nosema bombycis secretes serine protease inhibitor to suppress host cell apoptosis via Caspase BmICE.PLoS pathogens · 2025Article
- A novel ATP-binding cassette protein (NoboABCG1.3) plays a role in the proliferation of Nosema bombycis.Parasitology research · 2024Article
- Transgenic overexpression of bmo-miR-6498-5p increases resistance toApplied and environmental microbiology · 2024Article
- Treatment of MicrosporidiumMicroorganisms · 2024Article
- Functional characterization of Nosema bombycis (microsporidia) trehalase 3.Parasitology research · 2023Article
- Germination of Microsporidian Spores: The Known and Unknown.Journal of fungi (Basel, Switzerland) · 2023Review
- Construction of scFv Antibodies against the Outer Loops of the MicrosporidiumInternational journal of molecular sciences · 2022Article
- Heterologous Expressed NbSWP12 from MicrosporidiaJournal of fungi (Basel, Switzerland) · 2022Article
- Identification and subcellular localization analysis of membrane protein Ycf 1 in the microsporidianPeerJ · 2022Article
- Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nosema bombycis is a destructive, obligate intracellular parasite of the Bombyx mori. In this study, a single-chain variable fragment (scFv) dependent technology is developed for the purpose of inhibiting parasite proliferation in insect cells. The scFv-G4, which we prepared from a mouse G4 monoclonal antibody, can target the N. bombycis spore wall protein 12 (NbSWP12). Indirect immunofluorescence assays showed that NbSWP12 located mainly on the outside of the N. bombycis cytoskeleton, although some of it co-localized with β-tubulin in the meront-stage of parasites. When meront division began, NbSWP12 became concentrated at both ends of each meront. Western blotting showed that scFv-G4 could express in Sf9-III cells and recognized native NbSWP12. The transgenic Sf9-III cell line showed better resistance than the controls when challenged with N. bombycis, indicating that NbSWP12 is a promising target in this parasite and this scFv dependent strategy could be a solution for construction of N. bombycis-resistant Bombyx mori.
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