ArticleConnective tissue research2018
Release of pro-inflammatory cytokines from muscle and bone causes tenocyte death in a novel rotator cuff in vitro explant culture model.
Article in Connective tissue research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Alpha-2-Macroglobulin-Enriched Plasma Modulates Inflammatory and Proteolytic Pathways in Experimental Equine Musculoskeletal Models.Animals : an open access journal from MDPI · 2026Article
- Effect of human myoblasts on tenogenic progression in 2D and 3D culture models.Journal of anatomy · 2026Article
- Estrogen and Progesterone Exhibit Distinct Yet Coordinated Roles in the Regulation of Tendon Extracellular Matrix Remodeling.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Changes in gut microbiota and plasma metabolites in patients with symptomatic degenerative rotator cuff tendinopathy.Frontiers in microbiology · 2026Article
- Estrogen and progesterone exhibit distinct yet coordinated roles in the regulation of tendon extracellular matrix remodeling.bioRxiv : the preprint server for biology · 2025Article
- Enhanced rotator cuff tendon-bone interface regeneration with injectable manganese-based mesoporous silica nanoparticle-loaded dual crosslinked hydrogels.Frontiers in bioengineering and biotechnology · 2025Article
- Human Tendon-on-Chip: Unveiling the Effect of Core Compartment-T Cell Spatiotemporal Crosstalk at the Onset of Tendon Inflammation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Rheumatoid arthritis-recent advances in pathogenesis and the anti-inflammatory effect of plant-derived COX inhibitors.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Aged Tendons Exhibit Altered Mechanisms of Strain-Dependent Extracellular Matrix Remodeling.Journal of biomechanical engineering · 2024Article
- Understanding Tendon Fibroblast Biology and Heterogeneity.Biomedicines · 2024Review
- Murine Hind Limb Explant Model for Studying the Mechanobiology of Achilles Tendon Impingement.Journal of visualized experiments : JoVE · 2023Article
- Earlier proteoglycan turnover promotes higher efficiency matrix remodeling in MRL/MpJ tendons.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Dynamic Load Model Systems of Tendon Inflammation and Mechanobiology.Frontiers in bioengineering and biotechnology · 2022Review
- Anti-inflammatory and Tendon-Protective Effects of Adipose Stem Cell-Derived Exosomes with Concomitant Use of Glucocorticoids.Stem cells international · 2022Article
- Tendon Extracellular Matrix Assembly, Maintenance and Dysregulation Throughout Life.Advances in experimental medicine and biology · 2021Review
- Impact of Uniaxial Stretching on Both Gliding and Traction Areas of Tendon Explants in a Novel Bioreactor.International journal of molecular sciences · 2020Article
- Lose-Dose Administration of Dexamethasone Is Beneficial in Preventing Secondary Tendon Damage in a Stress-Deprived Joint Injury Explant Model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Age-associated changes in the response of tendon explants to stress deprivation is sex-dependent.Connective tissue research · 2020Article
- Calcaneal Tendon Plasticity Following Gastrocnemius Muscle Injury in Rat.Frontiers in physiology · 2019Article
- Skeletal muscle explants: ex-vivo models to study cellular behavior in a complex tissue environment.Connective tissue researchReview
Corrections and comments
- Erratum issuedCorrection.2019
Authors and funding
2 authors.
Funding
Abstract
purposeTendinopathy is a significant clinical problem thought to be associated with altered mechanical loading. Explant culture models allow researchers to alter mechanical loading in a controlled in vitro environment while maintaining tenocytes in their native matrix. However, current models do not accurately represent commonly injured tendons, ignoring contributions of associated musculature and bone, as well as regional collagen structure. This study details the characterization of amouse rotator cuff explant culture model, including bone, tendon, and muscle (BTM). MATERIALS AND
methodsFollowing harvest, BTM explants were maintained in stress-deprived culture for one week and tendon was then assessed for changes in cell viability, metabolism, matrix structure and content.
resultsMatrix turnover occurred throughout culture as manifested in both gene expression and biosynthesis, but this did not translate to net changes in total collagen or sulfated glycosaminoglycan content. Furthermore, tendon structure was not significantly altered throughout culture. However, we found significant cell death in BTM tendons after 3 days in culture, which we hypothesize is cytokine-induced. Using a targeted multiplex assay, we found high levels of pro-inflammatory cytokines released to the culture medium from muscle and bone, levels that did cause cell deathin tendon-alone controls.
conclusionsOverall, this model presents an innovative approach to understandingrotator cuff injury and tenocyte mechanobiology in a clinically-relevant tendon structure. Our model can be a powerful tool to investigate how mechanical and biological stimuli can alter normal tendon health and lead to tendon degeneration, and may provide a testbed for therapeutics for tendon repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.