Evidence map›Paper›PMID 29446054›Full record

ArticleNeurotoxicity research2018

Upregulation of Pro-inflammatory Cytokine Expression Following Chronic Paracetamol Treatment in Astrocyte.

Nutnicha Tantarungsee, Waranurin Yisarakun, Thananya Thongtan, Laddawan Lalert, Sirinapa Srikam, Preecha Reuangwechvorachai, Praewphan Ingruanglert, Supang Maneesri-le Grand

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In one paragraph

Article in Neurotoxicity research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nutnicha TantarungseeDepartment of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Waranurin YisarakunDepartment of Biomedical Sciences, Faculty of Allied Health Sciences, Burapha University, ChonBuri, 20131, Thailand.
Thananya ThongtanDepartment of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Laddawan LalertDepartment of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Sirinapa SrikamDepartment of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Preecha ReuangwechvorachaiDepartment of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Praewphan IngruanglertStem Cells and Cell Therapy Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
Supang Maneesri-le GrandDepartment of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. le.grand.maneesri.s@gmail.com.

Funding

National Research Council of Thailand GB_B_60_071_30_15 and GB_B_60_074_30_18
6 · The paper itself

Abstract

The present study aimed to investigate the effect of APAP treatment on the expression of pro-inflammatory cytokines in the astrocytes. The mouse astrocyte cells (C8-D1A) were treated with APAP at the concentration of 100 μM for 24 h, 16 and 28 days. The expressions of pro-inflammatory cytokines and NF-kB were determined using western blot analysis. Furthermore, the expression and localization of phosphorylation of NF-kB were detected by immunohistochemical and immunofluorescent analysis. The ultrastructure of C8-D1A cells was as well monitored. The results revealed that acute APAP treatment (24 h) had no effect on the expression of pro-inflammatory cytokines and pNF-kB. This treatment did not alter the ultrastructure of C8-D1A cells when compared with those in the control cells. However, the results obtained from the study on chronic APAP-treated cells (16 and 28 days) showed the different effect of APAP treatment. The results obtained from western blot analysis showed the increment of pro-inflammatory cytokine (IL-1β and TNF-α) expressions and the activation of NF-kB signaling pathway. Nuclear translocation of pNF-kB and alteration of several cell structures were well observed in the C8-D1A cells with chronic APAP treatment. The results obtained from this study suggest that chronic APAP treatment can induce an upregulation of pro-inflammatory cytokines (IL-1β and TNFα) in astrocytes. This alteration implies the involvement of the activation of NF-kB signaling pathway.

Indexed as

AcetaminophenAnalgesics, Non-NarcoticAnimalsAstrocytesCell Line, TransformedDose-Response Relationship, DrugInterleukin-1betaMiceNF-kappa BTime FactorsTumor Necrosis Factor-alphaUp-RegulationAcetaminophenAnalgesics, Non-NarcoticIL1B protein, mouseInterleukin-1betaNF-kappa BTumor Necrosis Factor-alphaAstrocyteNF-kB signalingParacetamolPro-inflammatory cytokinesUltrastructure

Identifiers

PMID29446054

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.