ArticleTransgenic research2018
Enhanced resistance to citrus canker in transgenic mandarin expressing Xa21 from rice.
Article in Transgenic research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Induction of Embryogenic Callus, Protoplast Isolation, and PEG-Mediated Transformation Protocols inPlants (Basel, Switzerland) · 2026Article
- Plant pattern recognition receptors: from evolutionary insight to engineering.Nature reviews. Genetics · 2025Review
- The Emerging Role ofPlants (Basel, Switzerland) · 2024Review
- Natural variation in the pattern-triggered immunity response in plants: Investigations, implications and applications.Molecular plant pathology · 2024Review
- Promoter characterization of a citrus linalool synthase gene mediating interspecific variation in resistance to a bacterial pathogen.BMC plant biology · 2023Article
- Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation.Nature communications · 2023Article
- Insights into the Molecular Basis of Huanglongbing Tolerance in Persian Lime (International journal of molecular sciences · 2023Article
- Citrus Canker Pathogen, Its Mechanism of Infection, Eradication, and Impacts.Plants (Basel, Switzerland) · 2022Review
- A prophage-encoded nonclassical secretory protein of "Candidatus Liberibacter asiaticus" induces a strong immune response in Nicotiana benthamiana and citrus.Molecular plant pathology · 2022Article
- Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
- RNA-Seq and Gene Regulatory Network Analyses Uncover Candidate Genes in the Early Defense to Two HemibiotrophicFrontiers in genetics · 2021Article
- CsPrx25, a class III peroxidase in Citrus sinensis, confers resistance to citrus bacterial canker through the maintenance of ROS homeostasis and cell wall lignification.Horticulture research · 2020Article
- Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.Plants (Basel, Switzerland) · 2020Review
- Heterologous Expression of thePlants (Basel, Switzerland) · 2020Article
- Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogen Xanthomonas.FEMS microbiology reviews · 2020Review
- New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review.Frontiers in plant science · 2020Review
- Citrus Genetic Engineering for Disease Resistance: Past, Present and Future.International journal of molecular sciences · 2019Review
- Establishment of Efficient Genetic Transformation Systems and Application of CRISPR/Cas9 Genome Editing Technology inInternational journal of molecular sciences · 2019Article
- Novel Plastid-Nuclear Genome Combinations Enhance Resistance to Citrus Canker in Cybrid Grapefruit.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic engineering approaches offer an alternative method to the conventional breeding of Citrus sp. 'W. Murcott' mandarin (a hybrid of 'Murcott' and an unknown pollen parent) is one of the most commercially important cultivars grown in many regions around the world. Transformation of 'W. Murcott' mandarin was achieved by direct DNA uptake using a protoplast transformation system. DNA construct (pAO3), encoding Green Fluorescent Protein (GFP) and the cDNA of Xa21, a Xanthomonas resistance gene from rice, was used to transform protoplasts of 'W. Murcott' mandarin. Following citrus protoplast culture and regeneration, transformed micro calli were microscopically designated via GFP expression, physically isolated from non-transformed tissue, and cultured on somatic embryogenesis induction medium. More than 150 transgenic embryos were recovered and from them, ten transgenic lines were regenerated and cultured on rooting medium for shoot elongation. Transgenic shoots were micrografted and established in the greenhouse with 3-5 replicates per line. The insertion of Xa21 and GFP was confirmed by PCR and southern blot analysis. GFP expression was verified by fluorescence microscopy and western blot analysis revealed expression of Xa21 although it was variable among transgenic lines, as shown by RT-qPCR. Transgenic plants challenged with the citrus canker pathogen by syringe inoculation showed a reduction in lesion number and bacterial populations within lesions compared to non-transgenic control plants. Transgenic 'W. Murcott' mandarin lines with improved canker resistance via protoplast transformation from embryogenic callus with the Xa21 gene from rice are being evaluated under field conditions to validate the level of resistance.
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