ArticleZoological letters2018
Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering.
Article in Zoological letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Structured spontaneous activity through delta oscillation-based discrete states in the medaka telencephalon.Scientific reports · 2026Article
- Comparative Analysis of Davidson and Glyoxal Fixatives on Autofluorescence and Immunolabeling in Medaka (Biomedicines · 2025Article
- Optimizing reproductive performance in pangasius catfish broodstock: A review of dietary and molecular strategies.Veterinary and animal science · 2024Review
- The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- An evolutionarily distinct Hmgn2 variant influences shape recognition in Medaka Fish.Communications biology · 2024Article
- Genetically engineered mouse model of HPV16 E6-E7 with vaginal-cervical intraepithelial neoplasia and decreased immunity.Heliyon · 2024Article
- A gene regulatory network combining Pax3/7, Sox10 and Mitf generates diverse pigment cell types in medaka and zebrafish.Development (Cambridge, England) · 2023Article
- High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes).BMC genomics · 2023Article
- Genetic manipulation of betta fish.bioRxiv : the preprint server for biology · 2023Article
- Genetic manipulation of betta fish.Frontiers in genome editing · 2023Article
- A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryos.Scientific reports · 2022Article
- Speciation and adaptation research meets genome editing.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging.Scientific reports · 2022Article
- Generation of knock-in lampreys by CRISPR-Cas9-mediated genome engineering.Scientific reports · 2021Article
- Manipulation of the Tyrosinase gene permits improved CRISPR/Cas editing and neural imaging in cichlid fish.Scientific reports · 2021Article
- CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome.Scientific reports · 2020Article
- Heat-shock-induced tyrosinase gene ablation with CRISPR in zebrafish.Molecular genetics and genomics : MGG · 2020Article
- [Generation of a novel HBeAg transgenic mice using CRISPR/Cas9 technique].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundMedaka (
resultsWe report the highly efficient generation of knock-in transgenic medaka via non-homologous end joining (NHEJ). Donor plasmid containing a heat-shock promoter and a reporter gene was co-injected with a short guide RNA (sgRNA) targeted for genome digestion, an sgRNA targeted for donor plasmid digestion, and Cas9 mRNA. Broad transgene expression in the expression domain of a target gene was observed in approximately 25% of injected embryos. By raising these animals, we established stable knock-in transgenic fish with several different constructs for five genetic loci, obtaining transgenic founders at efficiencies of > 50% for all five loci. Further, we show that the method is useful for obtaining mutant alleles. In the experiments where transgene integrations were targeted between the transcription start site and the initiation methionine, the resultant transgenic fish became mutant alleles.
conclusionWith its simplicity, design flexibility, and high efficiency, we propose that CRISPR/Cas9-mediated knock-in via NHEJ will become a standard method for the generation of transgenic and mutant medaka.
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