ArticleScientific reports2018
Development and application of a rapid Mycobacterium tuberculosis detection technique using polymerase spiral reaction.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Development and validation of P113 gene specific polymerase spiral reaction assay for rapid detection of Mycoplasma ovipneumoniae in sheep and goats.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Development and Application of a Polymerase Spiral Reaction (PSR)-Based Isothermal Assay for Rapid Detection of Yak (Foods (Basel, Switzerland) · 2025Article
- Unveiling the crucial genomic players in pulmonary tuberculosis-associated ferroptosis through bioinformatics scrutiny.BMC infectious diseases · 2025Article
- The Recent Development in the Diagnosis ofSmart medicine · 2025Review
- Peptide nucleic acid-immobilised paper combined with multiplex recombinase polymerase amplification for the ultrasensitive and rapid detection of rifampicin-resistant tuberculosis.Scientific reports · 2025Article
- Advances in Diagnostics and Drug Discovery against Resistant and Latent Tuberculosis Infection.Pharmaceutics · 2023Review
- A Novel and Rapid Isothermal Nucleic Acid Based Detection Assay ofIranian journal of public health · 2022Article
- Use of Digital Technology to Enhance Tuberculosis Control: Scoping Review.Journal of medical Internet research · 2020Article
- Polymerase Spiral Reaction Assay for Rapid and Real Time Detection of West Nile Virus From Clinical Samples.Frontiers in cellular and infection microbiology · 2020Article
- Novel polymerase spiral reaction assay for the visible molecular detection of porcine circovirus type 3.BMC veterinary research · 2019Article
- Click DNA cycling in combination with gold nanoparticles loaded with quadruplex DNA motifs enable sensitive electrochemical quantitation of the tuberculosis-associated biomarker CFP-10 in sputum.Mikrochimica acta · 2019Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium tuberculosis is an age-old bacterium that is difficult to eliminate. A simple and rapid diagnostic method is of great importance to prevent the spread of M. tuberculosis. Here, we developed a low-cost rapid M. tuberculosis nucleic acid detection technique, named GenePop, which enabled the storage and transport of M. tuberculosis diagnostic reagent at ambient temperatures, without the need for professional operations or expensive instrumentation. Using a vitrification method, we vitrified heat-unstable components onto the cap of a reaction tube, and placed heat-stable components at the bottom of the reaction tube by sealing them with paraffin wax. The all-in-one detection tube, when used together with our other invention-a multi-functional sample treatment tube pre-filled with a nucleic acid-releasing agent-only required three simple steps to yield results. A comparative analysis with a commercial qPCR kit for M. tuberculosis indicated that our new technique had a concordance rate of 91.6%, showing no cross-reactivity with 11 other bacteria. The complete operation time was only 65 min. It is suitable for use in field settings or by personnel in grass-root units, and is applicable in household activities, hence can be used in developing countries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.