ArticleScientific reports2018
Bronchoscopic fibered confocal fluorescence microscopy for longitudinal in vivo assessment of pulmonary fungal infections in free-breathing mice.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- An automated deep learning-based pipeline for 3D characterisation of the murine upper airway from micro-CT.Frontiers in medical technology · 2026Article
- Fungal infections in focus: accelerating non-invasive imaging from preclinical insights to clinical breakthroughs.Npj imaging · 2025Review
- Quantitative MRI of a Cerebral Cryptococcoma Mouse Model for In Vivo Distinction between Different Cryptococcal Molecular Types.Journal of fungi (Basel, Switzerland) · 2024Article
- Preclinical Models for Cryptococcosis of the CNS and Their Characterization Using In Vivo Imaging Techniques.Journal of fungi (Basel, Switzerland) · 2024Review
- The role of confocal laser endomicroscopy in pulmonary medicine.European respiratory review : an official journal of the European Respiratory Society · 2023Review
- Many ways, one microorganism: Several approaches to study Malassezia in interactions with model hosts.PLoS pathogens · 2022Review
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- From Mouse to Man and Back: Closing the Correlation Gap between Imaging and Histopathology for Lung Diseases.Diagnostics (Basel, Switzerland) · 2020Review
- Redundant Trojan horse and endothelial-circulatory mechanisms for host-mediated spread of Candida albicans yeast.PLoS pathogens · 2020Article
- Illuminating Macrophage Contributions to Host-Pathogen InteractionsInfection and immunity · 2020Review
- Let's shine a light on fungal infections: A noninvasive imaging toolbox.PLoS pathogens · 2020Review
- Analysis of factors of pulmonary fungal infection in mice in respiratory medicine department based on logistic regression analysis model and Progranulin.Saudi journal of biological sciences · 2020Article
- Longitudinal In Vivo Assessment of Host-Microbe Interactions in a Murine Model of Pulmonary Aspergillosis.iScience · 2019Article
- Sensitive bioluminescence imaging of fungal dissemination to the brain in mouse models of cryptococcosis.Disease models & mechanisms · 2019Article
- Yeast and Filaments Have Specialized, Independent Activities in a Zebrafish Model of Candida albicans Infection.Infection and immunity · 2018Article
- Methodologies forMicrobial cell (Graz, Austria) · 2018Review
- Advances in Optical Coherence Tomography and Confocal Laser Endomicroscopy in Pulmonary Diseases.Respiration; international review of thoracic diseasesReview
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Respiratory diseases, such as pulmonary infections, are an important cause of morbidity and mortality worldwide. Preclinical studies often require invasive techniques to evaluate the extent of infection. Fibered confocal fluorescence microscopy (FCFM) is an emerging optical imaging technique that allows for real-time detection of fluorescently labeled cells within live animals, thereby bridging the gap between in vivo whole-body imaging methods and traditional histological examinations. Previously, the use of FCFM in preclinical lung research was limited to endpoint observations due to the invasive procedures required to access lungs. Here, we introduce a bronchoscopic FCFM approach that enabled in vivo visualization and morphological characterisation of fungal cells within lungs of mice suffering from pulmonary Aspergillus or Cryptococcus infections. The minimally invasive character of this approach allowed longitudinal monitoring of infection in free-breathing animals, thereby providing both visual and quantitative information on infection progression. Both the sensitivity and specificity of this technique were high during advanced stages of infection, allowing clear distinction between infected and non-infected animals. In conclusion, our study demonstrates the potential of this novel bronchoscopic FCFM approach to study pulmonary diseases, which can lead to novel insights in disease pathogenesis by allowing longitudinal in vivo microscopic examinations of the lungs.
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