ArticleCell reports2018
Pak1 Kinase Maintains Apical Membrane Identity in Epithelia.
Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Deciphering the Impact of AKT1 Pathogenic Variants in Juvenile Granulosa Cell Tumors Using a Drosophila Model.Molecular & cellular proteomics : MCP · 2025Article
- Cell polarity proteins promote macropinocytosis in response to metabolic stress.Nature communications · 2024Article
- Apical polarity and actomyosin dynamics control Kibra subcellular localization and function in Drosophila Hippo signaling.Developmental cell · 2023Article
- Separable mechanisms drive local and global polarity establishment in the Caenorhabditiselegans intestinal epithelium.Development (Cambridge, England) · 2022Article
- Apical-basal polarity and the control of epithelial form and function.Nature reviews. Molecular cell biology · 2022Review
- GIANI - open-source software for automated analysis of 3D microscopy images.Journal of cell science · 2022Article
- Par-3 family proteins in cell polarity & adhesion.The FEBS journal · 2022Review
- Rac1 promotes kidney collecting duct integrity by limiting actomyosin activity.The Journal of cell biology · 2021Article
- Imaging therapeutic peptide transport across intestinal barriers.RSC chemical biology · 2021Review
- Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex.eLife · 2021Article
- RhoGAP19D inhibits Cdc42 laterally to control epithelial cell shape and prevent invasion.The Journal of cell biology · 2021Article
- Article
- Distinct activities of Scrib module proteins organize epithelial polarity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The cytoskeletal motor proteins Dynein and MyoV direct apical transport of Crumbs.Developmental biology · 2020Article
- Girdin is a component of the lateral polarity protein network restricting cell dissemination.PLoS genetics · 2020Article
- Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time.Developmental cell · 2020Article
- Review
- The dPix-Git complex is essential to coordinate epithelial morphogenesis and regulate myosin during Drosophila egg chamber development.PLoS genetics · 2019Article
- The Rho family GEF FARP2 is activated by aPKCι to control tight junction formation and polarity.Journal of cell science · 2019Article
- A chemical-genetics approach to study the role of atypical Protein Kinase C inDevelopment (Cambridge, England) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Epithelial cells are polarized along their apical-basal axis by the action of the small GTPase Cdc42, which is known to activate the aPKC kinase at the apical domain. However, loss of aPKC kinase activity was reported to have only mild effects on epithelial cell polarity. Here, we show that Cdc42 also activates a second kinase, Pak1, to specify apical domain identity in Drosophila and mammalian epithelia. aPKC and Pak1 phosphorylate an overlapping set of polarity substrates in kinase assays. Inactivating both aPKC kinase activity and the Pak1 kinase leads to a complete loss of epithelial polarity and morphology, with cells losing markers of apical polarization such as Crumbs, Par3/Bazooka, or ZO-1. This function of Pak1 downstream of Cdc42 is distinct from its role in regulating integrins or E-cadherin. Our results define a conserved dual-kinase mechanism for the control of apical membrane identity in epithelia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.