ArticlePlant & cell physiology2018
Structural and Functional Analysis of UGT92G6 Suggests an Evolutionary Link Between Mono- and Disaccharide Glycoside-Forming Transferases.
Article in Plant & cell physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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17 citing papers in PubMed, 26 citations in OpenAlex.
- Transcription Profiling of Glycosyltransferases inJournal of agricultural and food chemistry · 2025Article
- Kinetics of Secoisolariciresinol Glucosyltransferase LuUGT74S1 and Its Mutants.Journal of agricultural and food chemistry · 2024Article
- Subfunctionalization of a monolignol to a phytoalexin glucosyltransferase is accompanied by substrate inhibition.Plant communications · 2023Article
- Genome-wide identification of UDP-glycosyltransferases in the tea plant (Frontiers in plant science · 2023Review
- Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana.Communications biology · 2022Article
- UGT72, a Major Glycosyltransferase Family for Flavonoid and Monolignol Homeostasis in Plants.Biology · 2022Review
- Controllable Iterative β-Glucosylation from UDP-Glucose byJournal of agricultural and food chemistry · 2021Article
- Advances in steroidal saponins biosynthesis.Planta · 2021Review
- Glucosylation of (±)-Menthol by Uridine-Diphosphate-Sugar Dependent Glucosyltransferases from Plants.Molecules (Basel, Switzerland) · 2021Article
- A New Glycosyltransferase Enzyme from Family 91, UGT91P3, Is Responsible for the Final Glucosylation Step of Crocins in Saffron (International journal of molecular sciences · 2021Article
- Micropathogen community identification in ticks (Acari: Ixodidae) using third-generation sequencing.International journal for parasitology. Parasites and wildlife · 2021Article
- Characterization of the UDP-glycosyltransferase UGT72 Family in Poplar and Identification of Genes Involved in the Glycosylation of Monolignols.International journal of molecular sciences · 2020Article
- Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus.BMC plant biology · 2019Article
- Semirational design and engineering of grapevine glucosyltransferases for enhanced activity and modified product selectivity.Glycobiology · 2019Article
- Novel biotechnological glucosylation of high-impact aroma chemicals, 3(2H)- and 2(5H)-furanones.Scientific reports · 2019Article
- Glucosyltransferase CsUGT78A14 Regulates Flavonols Accumulation and Reactive Oxygen Species Scavenging in Response to Cold Stress inFrontiers in plant science · 2019Article
- Functional Differentiation of Duplicated Flavonoid 3-Frontiers in plant science · 2019Article
Corrections and comments
- Erratum issuedErratum.2018
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosylation mediated by UDP-dependent glycosyltransferase (UGT) is one of the most common reactions for the biosynthesis of small molecule glycosides. As glycosides have various biological roles, we characterized UGT genes from grapevine (Vitis vinifera). In silico analysis of VvUGT genes that were highly expressed in leaves identified UGT92G6 which showed sequence similarity to both monosaccharide and disaccharide glucoside-forming transferases. The recombinant UGT92G6 glucosylated phenolics, among them caffeic acid, carvacrol, eugenol and raspberry ketone, and also accepted geranyl glucoside and citronellyl glucoside. Thus, UGT92G6 formed mono- and diglucosides in vitro from distinct compounds. The enzyme specificity constant Vmax/Km ratios indicated that UGT92G6 exhibited the highest specificity towards caffeic acid, producing almost equal amounts of the 3- and 4-O-glucoside. Transient overexpression of UGT92G6 in Nicotiana benthamiana leaves confirmed the production of caffeoyl glucoside; however, the level of geranyl diglucoside was not elevated upon overexpression of UGT92G6, even after co-expression of genes encoding geraniol synthase and geraniol UGT to provide sufficient precursor. Comparative sequence and 3-D structure analysis identified a sequence motif characteristic for monoglucoside-forming UGTs in UGT92G6, suggesting an evolutionary link between mono- and disaccharide glycoside UGTs. Thus, UGT92G6 functions as a mono- and diglucosyltransferase in vitro, but acts as a caffeoyl glucoside UGT in N. benthamiana.
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