ArticlePloS one2018
An ancestral TMEM16 homolog from Dictyostelium discoideum forms a scramblase.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Copine A is essential for calcium homeostasis in Dictyostelium.Cell calcium · 2025Article
- Structure and Function of Calcium-Activated Chloride Channels and Phospholipid Scramblases in the TMEM16 Family.Handbook of experimental pharmacology · 2024Review
- Polymodal Control of TMEM16x Channels and Scramblases.International journal of molecular sciences · 2022Review
- The tertiary structure of the human Xkr8-Basigin complex that scrambles phospholipids at plasma membranes.Nature structural & molecular biology · 2021Article
- Ist2 recruits the lipid transporters Osh6/7 to ER-PM contacts to maintain phospholipid metabolism.The Journal of cell biology · 2021Article
- Phosphatidylserine exposure promotes increased adhesion in Dictyostelium Copine A mutants.PloS one · 2021Article
- TMEM16K is an interorganelle regulator of endosomal sorting.Nature communications · 2020Article
- Dynamic change of electrostatic field in TMEM16F permeation pathway shifts its ion selectivity.eLife · 2019Article
- Article
- Anoctamin 5/TMEM16E facilitates muscle precursor cell fusion.The Journal of general physiology · 2018Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
TMEM16 proteins are a recently identified protein family comprising Ca2+-activated Cl- channels that generate outwardly rectifying ionic currents in response to intracellular Ca2+ elevations. Some TMEM16 family members, such as TMEM16F/ANO6 are also essential for Ca2+-dependent phospholipid scrambling. TMEM16-like genes are present in the genomes of most eukaryotic species, the function(s) of TMEM16 family members from evolutionary ancient eukaryotes is not completely clear. Here, we provide insight into the evolution of these TMEM16 proteins by similarity searches for ancestral sequences. All eukaryotic genomes contain TMEM16 homologs, but only vertebrates have the full repertoire of ten distinct subtypes. TMEM16 homologs studied so far belong to the opisthokont branch of the phylogenetic tree, which includes the animal and fungal kingdoms. An organism outside this group is Dictyostelium discoideum, a representative of the amoebozoa group that diverged from the metazoa before fungi. We here functionally investigated the TMEM16 family member from Dictyostelium discoideum. When recombinantly expressed in HEK293 cells, DdTMEM16 induces phospholipid scrambling. However, in several electrophysiological experiments we did not find evidence for a Ca2+-activated Cl- channel function of DdTMEM16.
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