ArticlePloS one2018
Development and characterization of two cell lines from gills of Atlantic salmon.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Development and characterisation of a novel cell line derived from coho salmon (Oncorhynchus kisutch).In vitro cellular & developmental biology. Animal · 2026Article
- Myogenic Potential inACS omega · 2026Article
- Infection Dynamics and Host Responses to Two IPNV Isolates in Liver of Atlantic Salmon (Pathogens (Basel, Switzerland) · 2025Article
- Isolation and characterisation of two epithelial-like cell lines from the gills of Chrysophrys auratus (Australasian snapper) and Oncorhynchus tshawytscha (Chinook salmon) and their use in aquatic toxicology.In vitro cellular & developmental biology. Animal · 2025Article
- Atlantic Salmon Gill Epithelial Cell Line (ASG-10) as a Suitable Model for Xenobiotic Biotransformation.Metabolites · 2023Article
- Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing.BMC genomics · 2023Article
- The gill epithelial cell lines RTgill-W1, from Rainbow trout and ASG-10, from Atlantic salmon, exert different toxicity profiles towards rotenone.Cytotechnology · 2023Article
- Functional and molecular characterization of the Atlantic salmon gill epithelium cell line ASG-10; a tool forFrontiers in molecular biosciences · 2023Article
- Emergence of Salmon Gill Poxvirus.Viruses · 2022Review
- Development and characterization of a new gill cell line from the striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878).Fish physiology and biochemistry · 2022Article
- Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cells · 2021Article
- Differential Exoproteome and Biochemical Characterisation ofMicroorganisms · 2021Article
- Polarized Trout Epithelial Cells Regulate Transepithelial Electrical Resistance, Gene Expression, and the Phosphoproteome in Response to Viral Infection.Frontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cytokeratin and e-cadherin and with desmosomes as seen by transmission electron microscopy suggesting the cells to be of epithelial origin. These structures were not seen in ASG-13. The cell lines have been maintained for almost 30 passages and both cell lines are fully susceptible to infection by infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), Atlantic salmon reovirus TS (TSRV) and Pacific salmon paramyxovirus (PSPV). While infectious salmon anemia virus (ISAV) did not cause visible CPE, immunofluorescent staining revealed a sub-fraction of cells in both the ASG-10 and ASG-13 lines may be permissive to infection. ASG-10 is able to proliferate and migrate to close scratches in the monolayer within seven days in vitro contrary to ASG-13, which does not appear to do have the same proliferative and migratory ability. These cell lines will be useful in studies of gill diseases in Atlantic salmon and may represent an important contribution for alternatives to experimental animals and studies of epithelial-mesenchymal cell biology.
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