ArticleJournal of visualized experiments : JoVE2018
Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 16 citations in OpenAlex.
- Dynamic morphological staging of Drosophila pupal development.Scientific reports · 2026Article
- Thermal plasticity of wing size and wing spot size in Drosophila guttifera.Development genes and evolution · 2023Article
- The regulation of a pigmentation gene in the formation of complex color patterns in Drosophila abdomens.PloS one · 2022Article
- RNAMethods and protocols · 2021Article
- Revisiting the Role ofFrontiers in cell and developmental biology · 2021Article
- Evolution of wing pigmentation in Drosophila: Diversity, physiological regulation, and cis-regulatory evolution.Development, growth & differentiation · 2020Review
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diversified species of Drosophila (fruit fly) provide opportunities to study mechanisms of development and genetic changes responsible for evolutionary changes. In particular, the adult stage is a rich source of morphological traits for interspecific comparison, including wing pigmentation comparison. To study developmental differences among species, detailed observation and appropriate staging are required for precise comparison. Here we describe protocols for staging of pupal periods and quantification of wing pigmentation in a polka-dotted fruit fly, Drosophila guttifera. First, we describe the method for detailed morphological observation and definition of pupal stages based on morphologies. This method includes a technique for removing the puparium, which is the outer chitinous case of the pupa, to enable detailed observation of pupal morphologies. Second, we describe the method for measuring the duration of defined pupal stages. Finally, we describe the method for quantification of wing pigmentation based on image analysis using digital images and ImageJ software. With these methods, we can establish a solid basis for comparing developmental processes of adult traits during pupal stages.
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