ArticleJournal of visualized experiments : JoVE2018
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Fatty Acid Desaturases GmROD1s Are Involved in Nodulation by Regulating the Flux of Polyunsaturated Fatty Acids.Plant, cell & environment · 2026Article
- Optimization of CRISPR/Cas9-mediated CtPDS knockout in guar protoplasts.The plant genome · 2026Article
- Induction of Embryogenic Callus, Protoplast Isolation, and PEG-Mediated Transformation Protocols inPlants (Basel, Switzerland) · 2026Article
- Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method inJournal of fungi (Basel, Switzerland) · 2025Article
- Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Frontiers in plant science · 2025Article
- Isolation and transformation of perennial ryegrass (Frontiers in plant science · 2025Article
- DNA delivery into plant tissues using carbon dots made from citric acid and β-alanine.Frontiers in chemistry · 2025Article
- Advances in Soybean Genetic Improvement.Plants (Basel, Switzerland) · 2024Review
- Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli.Genes · 2024Review
- Soybean-Phakopsora pachyrhizi interactions: towards the development of next-generation disease-resistant plants.Plant biotechnology journal · 2024Review
- Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023Article
- Article
- Elucidating the Temporal Patterns of Gene Expression in the Inferred Regulatory Interactions ofmicroPublication biology · 2023Article
- Optimization of an Efficient Protoplast Transformation System for Transient Expression Analysis Using Leaves ofPlants (Basel, Switzerland) · 2022Article
- Functional Allele Validation by Gene Editing to Leverage the Wealth of Genetic Resources for Crop Improvement.International journal of molecular sciences · 2022Review
- Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen GeneInternational journal of molecular sciences · 2022Article
- Experimental Verification of Inferred Regulatory Interactions ofmicroPublication biology · 2022Article
- A DNA-Free Editing Platform for Genetic Screens in SoybeanFrontiers in plant science · 2022Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
- Optimizing RNAi-Target byFrontiers in plant science · 2021Article
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Authors and funding
2 authors.
Funding
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Abstract
Soybean (Glycine max (L.) Merr.) is an important crop species and has become a legume model for the studies of genetic and biochemical pathways. Therefore, it is important to establish an efficient transient gene expression system in soybean. Here, we report a simple protocol for the preparation of soybean protoplasts and its application for transient functional analyses. We found that young unifoliate leaves from soybean seedlings resulted in large quantities of high quality protoplasts. By optimizing a PEG-calcium-mediated transformation method, we achieved high transformation efficiency using soybean unifoliate protoplasts. This system provides an efficient and versatile model for examination of complex regulatory and signaling mechanisms in live soybean cells and may help to better understand diverse cellular, developmental and physiological processes of legumes.
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