ArticleJournal of visualized experiments : JoVE2018
Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- EMMIs: Engineered Myometrial Microtissues for Direct Quantification of Oxytocin-Induced Contractility.bioRxiv : the preprint server for biology · 2026Article
- Investigation of Uterine Contractility and Myometrial Stiffness in Pelvic Organ Prolapse.International journal of women's health · 2026Article
- Myometrial mechanotransduction and the timing of parturition: ion-channel pathways linking uterine stretch, calcium signaling, and inflammation.Frontiers in physiology · 2026Review
- Prolactin and oxytocin as modulators of intestinal contractility and glucose uptake.Frontiers in physiology · 2026Article
- Voltage-Gated Calcium Channels and the Parity-Dependent Differential Uterine Response to Oxytocin in Rats.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- A hydrogen sulphide-releasing non-steroidal anti-inflammatory, ATB-346, significantly attenuates human myometrial contractions.Pharmacological reports : PR · 2025Article
- Matrix Metallopeptidase 9 Promotes Contraction in Human Uterine Myometrium.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- The pig as a medical model for gynecological diseases: an applied perspective.Frontiers in veterinary science · 2025Review
- Contractile responses of engineered humanAPL bioengineering · 2024Article
- Maternal Body Mass Index, Myometrium Contractility and Uterotonic Receptor Expression in Pregnancy.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- BKCa channels are involved in spontaneous and lipopolysaccharide-stimulated uterine contraction in late gestation mice†.Biology of reproduction · 2024Article
- The Contractile Response to Oxytocin in Non-pregnant Rat Uteri Is Modified After the First Pregnancy.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- The In Vitro Contractile Response of Canine Pregnant Myometrium to Oxytocin and Denaverine Hydrochloride.Biology · 2023Article
- Assessment of the tocolytic nifedipine in preclinical primary models of preterm birth.Scientific reports · 2023Article
- Regulation of oxytocin-induced calcium transients and gene expression in engineered myometrial tissues by tissue architecture and matrix rigidity.Current research in physiology · 2023Article
- Review
- Dual effect of nifedipine on pregnant human myometrium contractility: Implication of TRPC1.Journal of cellular physiology · 2022Article
- Effects of Solvents, Emulsions, Cosolvents, and Complexions on Ex Vivo Mouse Myometrial Contractility.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Modeling and experimental approaches for elucidating multi-scale uterine smooth muscle electro- and mechano-physiology: A review.Frontiers in physiology · 2022Review
- Tools, techniques, and future opportunities for characterizing the mechanobiology of uterine myometrium.Experimental biology and medicine (Maywood, N.J.) · 2021Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We describe methods to measure ex vivo myometrium contractility. This assay can be used to investigate factors and molecules involved in the modulation of myometrial contraction and to determine their excitatory or inhibitory actions, and hence their therapeutic potential in vivo. Biopsies are obtained from women undergoing cesarean section delivery with informed consent. Fine strips of myometrium are dissected, clipped and attached to a force transducer within 1 mL organ baths superfused with physiological saline solution at 37 °C. Strips develop spontaneous contractions within 2-3 h under set tension and remain stable for many hours (>6 h). Strips can also be stimulated to contract such as by the endogenous hormones, oxytocin and vasopressin, which cause concentration-dependent modulation of contraction frequency, force and duration, to more closely resemble contractions in labor. Hence, the effect of known and novel drug leads can be tested on spontaneous and agonist-induced contractions. This protocol specifically details how this assay can be used to determine the potency of known and novel agents by measuring their effects on various parameters of human myometrial contraction. We use the oxytocin- and V1a receptor antagonists, atosiban and SR49059 as examples of known compounds which inhibit oxytocin- and vasopressin-induced contractions, and demonstrate how this method can be used to complement and validate pharmacological data obtained from cell-based assays to aid drug development. The effects of novel agonists in comparison to oxytocin and vasopressin can also be characterized. Whilst we use the example of the oxytocin/ vasopressin system, this method can also be used to study other receptors and ion channels that play a role in uterine contraction and relaxation to advance the understanding of human uterine physiology and pathophysiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.