Evidence map›Paper›PMID 29443027›Full record

ArticleJournal of visualized experiments : JoVE2018

Personalized Needles for Microinjections in the Rodent Brain.

Giovanna Paolone, Chiara Falcicchia, Gianluca Verlengia, Mario Barbieri, Anna Binaschi, Federico Paliotto, Beatrice Paradiso, Marie Soukupova, Silvia Zucchini, Michele Simonato

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PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Giovanna PaoloneDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara; giovanna.paolone@unife.it.
Chiara FalcicchiaDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara.
Gianluca VerlengiaDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara.
Mario BarbieriDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara.
Anna BinaschiDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara.
Federico PaliottoDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara.
Beatrice ParadisoDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara.
Marie SoukupovaDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara.
Silvia ZucchiniDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara; Laboratory for the Technologies for Advanced Therapies (LTTA), University of Ferrara.
Michele SimonatoDepartment of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences, University of Ferrara; Laboratory for the Technologies for Advanced Therapies (LTTA), University of Ferrara.
University of Ferrara · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microinjections have been used for a long time for the delivery of drugs or toxins within specific brain areas and, more recently, they have been used to deliver gene or cell therapy products. Unfortunately, current microinjection techniques use steel or glass needles that are suboptimal for multiple reasons: in particular, steel needles may cause tissue damage, and glass needles may bend when lowered deeply into the brain, missing the target region. In this article, we describe a protocol to prepare and use quartz needles that combine a number of useful features. These needles do not produce detectable tissue damage and, being very rigid, ensure reliable delivery in the desired brain region even when using deep coordinates. Moreover, it is possible to personalize the design of the needle by making multiple holes of the desired diameter. Multiple holes facilitate the injection of large amounts of solution within a larger area, whereas large holes facilitate the injection of cells. In addition, these quartz needles can be cleaned and re-used, such that the procedure becomes cost-effective.

Indexed as

NeedlesAnimalsBrainDisease Models, AnimalHumansMicroinjectionsPrecision MedicineRodentia

Identifiers

PMID29443027
PMCPMC5908696
OpenAlexW2784748325

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.