Evidence map›Paper›PMID 29443023›Full record

ArticleJournal of visualized experiments : JoVE2018

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage.

Xiangduo Kong, Gladys M S Cruz, Bárbara A Silva, Nicole M Wakida, Nima Khatibzadeh, Michael W Berns, Kyoko Yokomori

Open access · bronzeAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Poly-ADP-ribosylation dynamics, signaling, and analysis.Environmental and molecular mutagenesis · 2024
    Review
  3. Article
  4. Dissection of DNA damage and repair pathways in live cells by femtosecond laser microirradiation and free-electron modeling.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  5. Complex Chromatin Motions for DNA Repair.Frontiers in genetics · 2020
    Review
  6. Article
  7. Article
  8. In Time and Space: Laser Microirradiation and the DNA Damage Response.Methods in molecular biology (Clifton, N.J.) · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Xiangduo KongDepartment of Biological Chemistry, School of Medicine, University of California, Irvine.
Gladys M S CruzBeckman Laser Institute and Medical Clinic, University of California, Irvine.
Bárbara A SilvaBeckman Laser Institute and Medical Clinic, University of California, Irvine.
Nicole M WakidaBeckman Laser Institute and Medical Clinic, University of California, Irvine.
Nima KhatibzadehBeckman Laser Institute and Medical Clinic, University of California, Irvine.
Michael W BernsBeckman Laser Institute and Medical Clinic, University of California, Irvine; Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine; Department of Biomedical Engineering and Surgery, University of California, Irvine.
Kyoko YokomoriDepartment of Biological Chemistry, School of Medicine, University of California, Irvine; kyokomor@uci.edu.
University of California, Irvine · USBeckman Laser Institute and Medical Clinic · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA damage induces specific signaling and repair responses in the cell, which is critical for protection of genome integrity. Laser microirradiation became a valuable experimental tool to investigate the DNA damage response (DDR) in vivo. It allows real-time high-resolution single-cell analysis of macromolecular dynamics in response to laser-induced damage confined to a submicrometer region in the cell nucleus. However, various laser conditions have been used without appreciation of differences in the types of damage induced. As a result, the nature of the damage is often not well characterized or controlled, causing apparent inconsistencies in the recruitment or modification profiles. We demonstrated that different irradiation conditions (i.e., different wavelengths as well as different input powers (irradiances) of a femtosecond (fs) near-infrared (NIR) laser) induced distinct DDR and repair protein assemblies. This reflects the type of DNA damage produced. This protocol describes how titration of laser input power allows induction of different amounts and complexities of DNA damage, which can easily be monitored by detection of base and crosslinking damages, differential poly (ADP-ribose) (PAR) signaling, and pathway-specific repair factor assemblies at damage sites. Once the damage conditions are determined, it is possible to investigate the effects of different damage complexity and differential damage signaling as well as depletion of upstream factor(s) on any factor of interest.

Indexed as

DNA DamageLasersAnimalsDNA RepairHumans

Identifiers

PMID29443023
PMCPMC5912314
OpenAlexW2787337460

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.