Evidence map›Paper›PMID 29442248›Full record

ArticleExtremophiles : life under extreme conditions2018

The genes and enzymes of sucrose metabolism in moderately thermophilic methanotroph Methylocaldum szegediense O12.

Sergey Y But, Natalia P Solntseva, Svetlana V Egorova, Ildar I Mustakhimov, Valentina N Khmelenina, Alexander Reshetnikov, Yuri A Trotsenko

Abstract read
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In one paragraph

Article in Extremophiles : life under extreme conditions, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Sergey Y ButLaboratory of Methylotrophy, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russia. flash20063@rambler.ru.
Natalia P SolntsevaLaboratory of Methylotrophy, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russia.
Svetlana V EgorovaLaboratory of Methylotrophy, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russia.
Ildar I MustakhimovLaboratory of Methylotrophy, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russia.
Valentina N KhmeleninaLaboratory of Methylotrophy, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russia.
Alexander ReshetnikovLaboratory of Methylotrophy, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russia.
Yuri A TrotsenkoLaboratory of Methylotrophy, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russia.
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms · RU

Funding

Russian Foundation for Basic Research 16-04-00462-aRussian Scientific Foundation 18-14-00326
6 · The paper itself

Abstract

Four enzymes involved in sucrose metabolism: sucrose phosphate synthase (Sps), sucrose phosphate phosphatase (Spp), sucrose synthase (Sus) and fructokinase (FruK), were obtained as his-tagged proteins from the moderately thermophilic methanotroph Methylocaldum szegediense O12. Sps, Spp, FruK and Sus demonstrated biochemical properties similar to those of other bacterial counterparts, but the translated amino acid sequences of Sps and Spp displayed high divergence from the respective microbial enzymes. The Sus of M. szegediense O12 catalyzed the reversible reaction of sucrose cleavage in the presence of ADP or UDP and preferred ADP as a substrate, thus implying a connection between sucrose and glycogen metabolism. Sus-like genes were found only in a few methanotrophs, whereas amylosucrase was generally used in sucrose cleavage in this group of bacteria. Like other microbial fructokinases, FruK of M. szegediense O12 showed a high specificity to fructose.

Indexed as

Bacterial ProteinsFructokinasesGlucosyltransferasesMethylococcaceaePhosphoric Monoester HydrolasesSucroseBacterial ProteinsfructokinaseFructokinasesGlucosyltransferasesPhosphoric Monoester HydrolasesSucrosesucrose-phosphatasesucrose-phosphate synthasesucrose synthaseFructokinaseMethylocaldum szegedienseSucrose phosphate phosphataseSucrose phosphate synthaseSucrose synthase

Identifiers

PMID29442248
OpenAlexW2788221207

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.