ArticleScientific reports2018
Candidate-gene based GWAS identifies reproducible DNA markers for metabolic pyrethroid resistance from standing genetic variation in East African Anopheles gambiae.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 95 citations in OpenAlex.
- Effect of long-lasting insecticidal nets with and without piperonyl butoxide on malaria indicators in Uganda (LLINEUP): a pragmatic, cluster-randomised trial embedded in a national LLIN distribution campaign.Lancet (London, England) · 2020Trial
- Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.BMC genomics · 2026Article
- Spatial distribution of the 2La chromosomal inversion in Anopheles gambiae populations across Nigeria: ecological associations and implications for malaria control.Tropical medicine and health · 2026Article
- Genetic Surveillance Reveals Differential Evolutionary Dynamic of Anopheles gambiae Under Contrasting Insecticidal Tools Used in Malaria Control.Molecular ecology · 2026Article
- Article
- Genomic insights into clonal diversity in UK populations of the potato aphid, Macrosiphum euphorbiae.BMC genomics · 2025Article
- Chromosomal rearrangements in mosquitoes: from micro- to macroevolution.Current opinion in insect science · 2025Review
- The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner).PloS one · 2025Article
- LLIN Evaluation in Uganda Project (LLINEUP2) - Effect of long-lasting insecticidal nets (LLINs) treated with pyrethroid plus pyriproxyfen vs LLINs treated with pyrethroid plus piperonyl butoxide in Uganda: A cluster-randomised trial.PLOS global public health · 2025Article
- Cryptic population structure and insecticide resistance in Anopheles gambiae from the southern Democratic Republic of Congo.Scientific reports · 2024Article
- Mechanisms of transcriptional regulation inProceedings. Biological sciences · 2024Article
- Association of a rapidly selected 4.3kb transposon-containing structural variation with a P450-based resistance to pyrethroids in the African malaria vector Anopheles funestus.PLoS genetics · 2024Article
- LLIN Evaluation in Uganda Project (LLINEUP)-effects of a vector control trial on Plasmodium infection prevalence and genotypic markers of insecticide resistance in Anopheles vectors from 48 districts of Uganda.Scientific reports · 2024Article
- Signatures of adaptation at key insecticide resistance loci in Anopheles gambiae in Southern Ghana revealed by reduced-coverage WGS.Scientific reports · 2024Article
- Pyrethroid resistance and gene expression profile of a new resistantWellcome open research · 2024Article
- Behavioural responses ofCurrent research in parasitology & vector-borne diseases · 2024Article
- Mechanisms of transcriptional regulation inbioRxiv : the preprint server for biology · 2023Article
- Molecular drivers of insecticide resistance in the Sahelo-Sudanian populations of a major malaria vector Anopheles coluzzii.BMC biology · 2023Article
- RNA-Seq-Pop: Exploiting the sequence in RNA sequencing-A Snakemake workflow reveals patterns of insecticide resistance in the malaria vector Anopheles gambiae.Molecular ecology resources · 2023Article
- Characterizing pyrethroid resistance and mechanisms inCurrent research in parasitology & vector-borne diseases · 2023Article
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Authors and funding
13 authors at 8 institutions in 5 countries.
Funding
Abstract
Metabolic resistance to pyrethroid insecticides is widespread in Anopheles mosquitoes and is a major threat to malaria control. DNA markers would aid predictive monitoring of resistance, but few mutations have been discovered outside of insecticide-targeted genes. Isofemale family pools from a wild Ugandan Anopheles gambiae population, from an area where operational pyrethroid failure is suspected, were genotyped using a candidate-gene enriched SNP array. Resistance-associated SNPs were detected in three genes from detoxification superfamilies, in addition to the insecticide target site (the Voltage Gated Sodium Channel gene, Vgsc). The putative associations were confirmed for two of the marker SNPs, in the P450 Cyp4j5 and the esterase Coeae1d by reproducible association with pyrethroid resistance in multiple field collections from Uganda and Kenya, and together with the Vgsc-1014S (kdr) mutation these SNPs explained around 20% of variation in resistance. Moreover, the >20 Mb 2La inversion also showed evidence of association with resistance as did environmental humidity. Sequencing of Cyp4j5 and Coeae1d detected no resistance-linked loss of diversity, suggesting selection from standing variation. Our study provides novel, regionally-validated DNA assays for resistance to the most important insecticide class, and establishes both 2La karyotype variation and humidity as common factors impacting the resistance phenotype.
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