ArticleScientific reports2018
A chemoenzymatic process for amide bond formation by an adenylating enzyme-mediated mechanism.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Papain-Mediated Conjugation of Peptide Nucleic Acids to Delivery Peptides: A Density Functional Theory/Molecular Mechanics Metadynamics Study in Aqueous and Organic Solvent.The journal of physical chemistry. B · 2024Article
- Broad Spectrum Enantioselective Amide Bond Synthetase fromACS catalysis · 2024Article
- Non-canonical two-step biosynthesis of anti-oomycete indole alkaloids in Kickxellales.Fungal biology and biotechnology · 2023Article
- One-pot synthesis of 2,5-diketopiperazine with high titer and versatility using adenylation enzyme.Applied microbiology and biotechnology · 2022Article
- Gut Microbiome and Degradation Product Formation during Biodegradation of Expanded Polystyrene by Mealworm Larvae under Different Feeding Strategies.Molecules (Basel, Switzerland) · 2021Article
- Enzymatic Late-Stage Modifications: Better Late Than Never.Angewandte Chemie (International ed. in English) · 2021Review
- Enzymkatalysierte späte Modifizierungen: Besser spät als nie.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Review
- Article
- The Biosynthesis and Metabolism of theFrontiers in molecular biosciences · 2021Review
- Polystyrene Biodegradation byPolymers · 2020Article
- Diamine Biosynthesis: Research Progress and Application Prospects.Applied and environmental microbiology · 2020Review
- Biocatalytic Synthesis of Moclobemide Using the Amide Bond Synthetase McbA Coupled with an ATP Recycling System.ACS catalysis · 2020Article
- HAMA: a multiplexed LC-MS/MS assay for specificity profiling of adenylate-forming enzymes.Chemical science · 2019Article
- Synthesis of d-Amino Acid-Containing Dipeptides Using the Adenylation Domains of Nonribosomal Peptide Synthetase.Applied and environmental microbiology · 2019Article
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4 authors.
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Abstract
Amide bond formation serves as a fundamental reaction in chemistry, and is practically useful for the synthesis of peptides, food additives, and polymers. However, current methods for amide bond formation essentially generate wastes and suffer from poor atom economy under harsh conditions. To solve these issues, we demonstrated an alternative synthesis method for diverse tryptophyl-N-alkylamides by the combination of the first adenylation domain of tyrocidine synthetase 1 with primary or secondary amines as nucleophiles. Moreover, the physiological role of this domain is L-phenylalanine adenylation; however, we revealed that it displayed broad substrate flexibility from mono-substituted tryptophan analogues to even D-tryptophan. To the best of our knowledge, this is the first evidence for an adenylating enzyme-mediated direct amide bond formation via a sequential enzymatic activation of amino acids followed by nucleophilic substitution by general amines. These findings facilitate the design of a promising tool for biocatalytic straightforward amide bond formation with less side products.
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