ArticleNature communications2018
Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Ubiquitin-dependent mitochondrial protein degradation ensures seedling emergence by regulating ER-mitochondrial interaction and mitophagy.Nature plants · 2026Article
- SNX-mediated biogenesis of a plant-unique vesicle derived from the multivesicular body.Nature communications · 2026Article
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity.Nature plants · 2025Article
- NpCIPK6-NpSnRK1 module facilitates intersubgeneric hybridization barriers in water lily (Horticulture research · 2025Article
- Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling.Nature communications · 2023Article
- Research Progresses and Applications of Fluorescent Protein Antibodies: A Review Focusing on Nanobodies.International journal of molecular sciences · 2023Review
- A plant-unique protein BLISTER coordinates with core retromer to modulate endosomal sorting of plasma membrane and vacuolar proteins.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- An unexpected function for an ESCRT protein.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components.The Plant cell · 2022Article
- Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural insights into how vacuolar sorting receptors recognize the sorting determinants of seed storage proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Turnip mosaic virus co-opts the vacuolar sorting receptor VSR4 to promote viral genome replication in plants by targeting viral replication vesicles to the endosome.PLoS pathogens · 2022Article
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization.The Plant cell · 2021Article
- Motif-based endomembrane trafficking.Plant physiology · 2021Article
- Nanobody-Dependent Delocalization of Endocytic Machinery inFrontiers in plant science · 2021Article
- Applications of nanobodies in plant science and biotechnology.Plant molecular biology · 2021Review
- EMAC, Retromer, and VSRs: do they connect?Protoplasma · 2020Article
- On the discovery of an endomembrane compartment in plants.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein degradation in lytic compartments is crucial for eukaryotic cells. At the heart of this process, vacuolar sorting receptors (VSRs) bind soluble hydrolases in the secretory pathway and release them into the vacuolar route. Sorting efficiency is suggested to result from receptor recycling. However, how and to where plant VSRs recycle remains controversial. Here we present a nanobody-epitope interaction-based protein labeling and tracking approach to dissect their anterograde and retrograde transport routes in vivo. We simultaneously employ two different nanobody-epitope pairs: one for the location-specific post-translational fluorescence labeling of receptors and the other pair to trigger their compartment-specific lockdown via an endocytosed dual-epitope linker protein. We demonstrate VSR recycling from the TGN/EE, thereby identifying the cis-Golgi as the recycling target and show that recycled VSRs reload ligands. This is evidence that bidirectional VSR-mediated sorting of vacuolar proteins exists and occurs between the Golgi and the TGN/EE.
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