ArticleScientific reports2018
Rhamnolipids production from sucrose by engineered Saccharomyces cerevisiae.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Engineering growth independent production: programmable control loops for high-titer rhamnolipids for improved green surfactant sustainability.Bioprocess and biosystems engineering · 2026Review
- Purification, Characterization, and Oil-Displacement Performance of Rhamnolipid Biosurfactant Produced byJournal of microbiology and biotechnology · 2026Article
- Dirhamnolipids fromACS omega · 2026Article
- Multidomain DNA-Protein Mining Reveals Polymorphic Variations in RhlB Enhancing Monorhamnolipid Biosynthesis.ACS synthetic biology · 2026Article
- Reconstruction and exploitation of a dedicated Genome-Scale Metabolic Model of the human pathogenbioRxiv : the preprint server for biology · 2025Article
- Antioxidant Properties of Biosurfactants: Multifunctional Biomolecules with Added Value in Formulation Chemistry.Biomolecules · 2025Review
- Unveiling new features of the human pathogenComputational and structural biotechnology journal · 2025Article
- A review of yeast-derived emulsifiers developed through microbial fermentation for the food sector.Frontiers in microbiology · 2025Review
- Prospects and challenges regarding biosurfactants in advancing the petroleum industry.Frontiers in bioengineering and biotechnology · 2025Review
- Rhamnolipids bio-production and miscellaneous applications towards green technologies: a literature review.PeerJ · 2025Review
- Improving rhamnolipids production using fermentation-foam fractionation coupling system: cell immobilization and waste frying oil emulsion.Bioprocess and biosystems engineering · 2023Article
- Glycolipid Biosurfactants in Skincare Applications: Challenges and Recommendations for Future Exploitation.Molecules (Basel, Switzerland) · 2023Review
- Process Development in Biosurfactant Production.Advances in biochemical engineering/biotechnology · 2022Review
- Fungal biosurfactants, from nature to biotechnological product: bioprospection, production and potential applications.Bioprocess and biosystems engineering · 2021Review
- Yeast Synthetic Biology for the Production ofMolecules (Basel, Switzerland) · 2021Review
- Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico.Marine biotechnology (New York, N.Y.) · 2021Article
- Importance of the CMolecules (Basel, Switzerland) · 2020Article
- Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery.Microorganisms · 2020Article
- Heterologous Rhamnolipid Biosynthesis: Advantages, Challenges, and the Opportunity to Produce Tailor-Made Rhamnolipids.Frontiers in bioengineering and biotechnology · 2020Review
- Heterologous Expression of Sfp-Type Phosphopantetheinyl Transferase is Indispensable in the Biosynthesis of Lipopeptide Biosurfactant.Molecular biotechnology · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biosurfactants are biological tensioactive agents that can be used in the cosmetic and food industries. Rhamnolipids are glycolipid biosurfactants naturally produced by Pseudomonas aeruginosa and are composed of one or two rhamnose molecules linked to beta-hydroxy fatty acid chains. These compounds are green alternatives to petrochemical surfactants, but their large-scale production is still in its infancy, hindered due to pathogenicity of natural producer, high substrate and purification costs and low yields and productivities. This study, for the first time, aimed at producing mono-rhamnolipids from sucrose by recombinant GRAS Saccharomyces cerevisiae strains. Six enzymes from P. aeruginosa involved in mono-rhamnolipid biosynthesis were functionally expressed in the yeast. Furthermore, its SUC2 invertase gene was disrupted and a sucrose phosphorylase gene from Pelomonas saccharophila was also expressed to reduce the pathway's overall energy requirement. Two strains were constructed aiming to produce mono-rhamnolipids and the pathway's intermediate dTDP-L-rhamnose. Production of both molecules was analyzed by confocal microscopy and mass spectrometry, respectively. These strains displayed, for the first time as a proof of concept, the potential of production of these molecules by a GRAS eukaryotic microorganism from an inexpensive substrate. These constructs show the potential to further improve rhamnolipids production in a yeast-based industrial bioprocess.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.