ArticleProceedings of the National Academy of Sciences of the United States of America2018
Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Detection of Target Molecules Within One-Millimeter-Thick Mouse Brain Slices by Using Peroxidase-Fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Reaction.Bio-protocol · 2026Article
- Magnetic nanocomposite-based dual-readout lateral flow biosensor for sensitive and point-of-care detection of Burkholderia gladioli pv. cocovenenans in rice and milk products.Mikrochimica acta · 2025Article
- Multifunctional Nanobody Fusion Proteins in Immunoassays: Diverse Strategies for Enhanced Analytical Performance.Trends in analytical chemistry : TRAC · 2025Article
- Pentameric nanobodies serve as a capture agent and RANbodies function as immunoprobes for the sensitive detection of Salmonella typhimurium in immunoassays.Analytical and bioanalytical chemistry · 2025Article
- A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification.Communications biology · 2025Article
- A novel PolyAr87-based cell transfection protocol for nanobody expression optimized via a targeted design of transfection approach.Frontiers in bioengineering and biotechnology · 2025Article
- Production of high-affinity glycosylated anti-mouse conjugated nanobodies inFrontiers in bioengineering and biotechnology · 2025Article
- Article
- Plant virus-derived nanoparticles decorated with genetically encoded SARS-CoV-2 nanobodies display enhanced neutralizing activity.Plant biotechnology journal · 2024Article
- Plant-derived foot-and-mouth disease virus antibodies fused to horseradish peroxidase are efficient diagnostic reagents.Plant biotechnology journal · 2024Article
- Chimeric Protein Switch Biosensors.Advances in biochemical engineering/biotechnology · 2024Review
- Teneurin-3 regulates the generation of non-image-forming visual circuitry and responsiveness to light in the suprachiasmatic nucleus.PLoS biology · 2023Article
- Identification of a Linear B Cell Epitope on p54 of African Swine Fever Virus Using Nanobodies as a Novel Tool.Microbiology spectrum · 2023Article
- Sortase A transpeptidation produces seamless, unbranched biotinylated nanobodies for multivalent and multifunctional applications.Nanoscale advances · 2023Article
- Research Progresses and Applications of Fluorescent Protein Antibodies: A Review Focusing on Nanobodies.International journal of molecular sciences · 2023Review
- Construction of Synthetic VHH Libraries in Ribosome Display Format.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Characterization of non-alpha retinal ganglion cell injury responses reveals a possible block to restoring ipRGC function.Experimental neurology · 2022Article
- Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals.Communications biology · 2022Article
- A Novel Nanobody-Horseradish Peroxidase Fusion Based-Competitive ELISA to Rapidly Detect Avian Corona-Virus-Infectious Bronchitis Virus Antibody in Chicken Serum.International journal of molecular sciences · 2022Article
- HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies.Applied microbiology and biotechnology · 2022Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Sensitive and specific antibodies are essential for detecting molecules in cells and tissues. However, currently used polyclonal and monoclonal antibodies are often less specific than desired, difficult to produce, and available in limited quantities. A promising recent approach to circumvent these limitations is to employ chemically defined antigen-combining domains called "nanobodies," derived from single-chain camelid antibodies. Here, we used nanobodies to prepare sensitive unimolecular detection reagents by genetically fusing cDNAs encoding nanobodies to enzymatic or antigenic reporters. We call these fusions between a reporter and a nanobody "RANbodies." They can be used to localize epitopes and to amplify signals from fluorescent proteins. They can be generated and purified simply and in unlimited amounts and can be preserved safely and inexpensively in the form of DNA or digital sequence.
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Registered trials
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