ArticleBMC genomics2018
High fidelity hypothermic preservation of primary tissues in organ transplant preservative for single cell transcriptome analysis.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Robust single nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity.bioRxiv : the preprint server for biology · 2024Article
- Supercooled preservation of cultured primary rat hepatocyte monolayers.Frontiers in bioengineering and biotechnology · 2024Article
- Multi-omics Approach in Kidney Transplant: Lessons Learned from COVID-19 Pandemic.Current transplantation reports · 2023Article
- Sample Collection Methods in Upper Gastrointestinal Research.Journal of Korean medical science · 2023Review
- Transitioning single-cell genomics into the clinic.Nature reviews. Genetics · 2023Review
- Practical Considerations for Single-Cell Genomics.Current protocols · 2022Review
- Single-cell transcriptomics reveals expression profiles of Trypanosoma brucei sexual stages.PLoS pathogens · 2022Article
- Advances in single-cell sequencing: insights from organ transplantation.Military Medical Research · 2021Review
- Advanced technologies for the preservation of mammalian biospecimens.Nature biomedical engineering · 2021Review
- Current Methodological Challenges of Single-Cell and Single-Nucleus RNA-Sequencing in Glomerular Diseases.Journal of the American Society of Nephrology : JASN · 2021Review
- Enabling Technologies for Personalized and Precision Medicine.Trends in biotechnology · 2020Review
- Article
- scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation.Genome biology · 2019Article
- Article
- Tutorial: guidelines for the experimental design of single-cell RNA sequencing studies.Nature protocols · 2018Review
- The Human Cell Atlas: Technical approaches and challenges.Briefings in functional genomics · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundHigh-fidelity preservation strategies for primary tissues are in great demand in the single cell RNAseq community. A reliable method would greatly expand the scope of feasible multi-site collaborations and maximize the utilization of technical expertise. When choosing a method, standardizability and fidelity are important factors to consider due to the susceptibility of single-cell RNAseq analysis to technical noise. Existing approaches such as cryopreservation and chemical fixation are less than ideal for failing to satisfy either or both of these standards.
resultsHere we propose a new strategy that leverages preservation schemes developed for organ transplantation. We evaluated the strategy by storing intact mouse kidneys in organ transplant preservative solution at hypothermic temperature for up to 4 days (6 h, 1, 2, 3, and 4 days), and comparing the quality of preserved and fresh samples using FACS and single cell RNAseq. We demonstrate that the strategy effectively maintained cell viability, transcriptome integrity, cell population heterogeneity, and transcriptome landscape stability for samples after up to 3 days of preservation. The strategy also facilitated the definition of the diverse spectrum of kidney resident immune cells, to our knowledge the first time at single cell resolution.
conclusionsHypothermic storage of intact primary tissues in organ transplant preservative maintains the quality and stability of the transcriptome of cells for single cell RNAseq analysis. The strategy is readily generalizable to primary specimens from other tissue types for single cell RNAseq analysis.
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