ArticleInternational journal of molecular sciences2018
Determination of Proteinaceous Selenocysteine in Selenized Yeast.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 20 citations in OpenAlex.
- Biotransformation of Selenium in Yeast-Speciation and Nanostructural Analysis.Biological trace element research · 2026Article
- Review
- Extraction recovery and speciation of selenium in Se-enriched yeast.Analytical and bioanalytical chemistry · 2024Article
- Inorganic and Organic Selenium Speciation of Seleno-Yeasts Used as Feed Additives: New Insights from Elemental Selenium Determination.Biological trace element research · 2023Article
- Scientific opinion on the tolerable upper intake level for selenium.EFSA journal. European Food Safety Authority · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method for the quantitation of proteinaceous selenocysteine (SeCys) in Se-rich yeast was developed. The method is based on the reduction of the Se-Se and S-Se bridges with dithiotretiol, derivatization with iodoacetamide (carbamidomethylation), followed by HPLC-ICP MS. The chromatographic conditions were optimized for the total recovery of the proteinaceous selenocysteine, the minimum number of peaks in the chromatogram (reduction of derivatization products of other Se-species present) and the baseline separation. A typical chromatogram of a proteolytic digest of selenized yeast protein consisted of up to five peaks (including SeMet, carbamidomethylated (CAM)-SeCys, and Se(CAM)₂) identified by retention time matching with available standards and electrospray MS. Inorganic selenium non-specifically attached to proteins and selenomethionine could be quantified (in the form of Se(CAM)₂) along with SeCys. Selenocysteine, selenomethionine, inorganic selenium, and the water soluble-metabolite fraction accounted for the totality of selenium species in Se-rich yeast.
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Registered trials
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