ArticlePloS one2018
CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 32 citations in OpenAlex.
- A comprehensive review of CRISPR-Cas9-mediated genome editing in Leishmania strains: methodologies, applications, challenges and future directions.Molecular biology reports · 2026Review
- Queuosine tRNA modification regulates translational adaptation and virulence of Leishmania mexicana.PLoS biology · 2026Article
- Genetic Manipulation Tools in Leishmania: From CRISPR/Cas9 to Vaccine Strategies for Disease Control.Acta parasitologica · 2025Review
- Leishmania mexicana telomeres at high resolution: Ku80, TERT, and alternative lengthening mechanisms.BMC genomics · 2025Article
- Gunacins: Novel Benzo[ACS omega · 2025Article
- A novel strain of Leishmania braziliensis harbors not a toti- but a bunyavirus.PLoS neglected tropical diseases · 2024Article
- Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Leishmania Strains.Acta parasitologica · 2024Review
- From classical approaches to new developments in genetic engineering of live attenuated vaccine against cutaneous leishmaniasis: potential and immunization.Frontiers in public health · 2024Review
- Elimination of LRVs Elicits Different Responses inmSphere · 2022Article
- Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content.PLoS neglected tropical diseases · 2022Article
- Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis.NPJ vaccines · 2022Article
- Ku80 is involved in telomere maintenance but dispensable for genomic stability in Leishmania mexicana.PLoS neglected tropical diseases · 2021Article
- Catalase impairsVirulence · 2021Article
- Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?Pathogens (Basel, Switzerland) · 2021Review
- A New Model Trypanosomatid,mBio · 2021Article
- Targeted Deletion of Centrin inFrontiers in cellular and infection microbiology · 2021Article
- Nuclear and mitochondrial genome sequencing of North-AfricanMicrobial genomics · 2020Article
- Article
- The First Non-LRV RNA Virus inViruses · 2020Article
- Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmania parasites cause human cutaneous, mucocutaneous and visceral leishmaniasis. Several studies proposed involvement of certain genes in infectivity of these parasites based on differential mRNA expression data. Due to unusual gene expression mechanism, functions of such genes must be further validated experimentally. Here, we investigated a role of one of the putative virulence factors, LmxM.22.0010-encoded BTN1 (a protein involved in Batten disease in humans), in L. mexicana infectivity. Due to the incredible plasticity of the L. mexicana genome, we failed to obtain a complete knock-out of LmxM.22.0010 using conventional recombination-based approach even after ablating four alleles of this gene. To overcome this, we established a modified CRISPR-Cas9 system with genomic expression of Cas9 nuclease and gRNA. Application of this system allowed us to establish a complete BTN1 KO strain of L. mexicana. The mutant strain did not show any difference in growth kinetics and differentiation in vitro, as well as in the infectivity for insect vectors and mice hosts. Based on the whole-transcriptome profiling, LmxM.22.0010-encoded BTN1 was considered a putative factor of virulence in Leishmania. Our study suggests that ablation of LmxM.22.0010 does not influence L. mexicana infectivity and further illustrates importance of experimental validation of in silico-predicted virulence factors. Here we also describe the whole genome sequencing of the widely used model isolate L. mexicana M379 and report a modified CRISPR/Cas9 system suitable for complete KO of multi-copy genes in organisms with flexible genomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.