ArticleSensors (Basel, Switzerland)2018
Hydrogel-Based Fluorescent Dual pH and Oxygen Sensors Loaded in 96-Well Plates for High-Throughput Cell Metabolism Studies.
Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Oxygels: Tunable Phosphorescent Hydrogels for Cherenkov-Excited Luminescence Imaging (CELI) of Oxygen.Advanced healthcare materials · 2026Article
- Establishing a 3D Vascularized Tri-Culture Model of the Human Airways via a Digital Light Processing Bioprinter.Biotechnology and bioengineering · 2025Article
- Towards homogenous multiwell plate based pH sensors using a responsive triangulenium dye and an ATTO-647 reference dye.RSC advances · 2025Article
- Cy5 Dye Cassettes Exhibit Through-Bond Energy Transfer and Enable Ratiometric Fluorescence Sensing.The Journal of organic chemistry · 2024Article
- High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing.Frontiers in bioengineering and biotechnology · 2023Article
- Antimicrobial Efficiency of Chitosan and Its Methylated Derivative againstMolecules (Basel, Switzerland) · 2022Article
- A Dual pH/OMolecules (Basel, Switzerland) · 2022Article
- Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models.Sensors (Basel, Switzerland) · 2022Review
- Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.Mikrochimica acta · 2021Article
- Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity.Biomedical microdevices · 2020Article
- Autonomous and In Situ Ocean Environmental Monitoring on Optofluidic Platform.Micromachines · 2020Review
- Review of Dissolved Oxygen Detection Technology: From Laboratory Analysis to Online Intelligent Detection.Sensors (Basel, Switzerland) · 2019Review
- Design of a multi-sensor platform for integrating extracellular acidification rate with multi-metabolite flux measurement for small biological samples.Biosensors & bioelectronics · 2019Article
- Photons Probe Entropic Potential Variation during Molecular Confinement in Nanocavities.Entropy (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we developed fluorescent dual pH and oxygen sensors loaded in multi-well plates for in-situ and high-throughput monitoring of oxygen respiration and extracellular acidification during microbial cell growth for understanding metabolism. Biocompatible PHEMA-co-PAM materials were used as the hydrogel matrix. A polymerizable oxygen probe (OS2) derived from PtTFPP and a polymerizable pH probe (S2) derived from fluorescein were chemically conjugated into the matrix to solve the problem of the probe leaching from the matrix. Gels were allowed to cure directly on the bottom of 96-well plates at room-temperature via redox polymerization. The influence of matrix's composition on the sensing behaviors was investigated to optimize hydrogels with enough robustness for repeatable use with good sensitivity. Responses of the dual sensing hydrogels to dissolved oxygen (DO) and pH were studied. These dual oxygen-pH sensing plates were successfully used for microbial cell-based screening assays, which are based on the measurement of fluorescence intensity changes induced by cellular oxygen consumption and pH changes during microbial growth. This method may provide a real-time monitoring of cellular respiration, acidification, and a rapid kinetic assessment of multiple samples for cell viability as well as high-throughput drug screening. All of these assays can be carried out by a conventional plate reader.
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