ArticleInternational journal of molecular medicine2018
Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia.
Article in International journal of molecular medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
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- The Tumor-Promoting Role of ADM in Bladder Cancer and its Mediated Mechanisms of Cell Death via PKR and STAT1.Applied biochemistry and biotechnology · 2026Article
- Curcumin Protected CoClFood science & nutrition · 2026Article
- The molecular mechanisms and therapeutic implications of PANoptosis in ischemic diseases.Apoptosis : an international journal on programmed cell death · 2025Review
- Lactate inhibits mouse embryonic palatal mesenchyme cells chondrogenesis through AKT-PKM2 axis.BMC oral health · 2025Article
- Toll-Like Receptor 4-Mediated Neuroinflammation: Updates on Pathological Roles and Therapeutic Strategies in Chronic Cerebral Hypoperfusion.Molecular neurobiology · 2025Review
- Targeting the ERK1/2 and p38 MAPK pathways attenuates Golgi tethering factor golgin-97 depletion-induced cancer progression in breast cancer.Cell communication and signaling : CCS · 2025Article
- 6-hydroxygenistein attenuates hypoxia-induced injury via activating Nrf2/HO-1 signaling pathway in PC12 cells.Scientific reports · 2025Article
- Hypoxia regulates glycolysis through the HIF-1α/BMAL1/ALDOC axis to reduce oxaliplatin sensitivity in colorectal cancer.Journal of Cancer · 2025Article
- Article
- Sperm freezing damage: the role of regulated cell death.Cell death discovery · 2024Review
- Pathophysiological changes of muscle after ischemic stroke: a secondary consequence of stroke injury.Neural regeneration research · 2024Review
- Necrostatin-1: a promising compound for neurological disorders.Frontiers in cellular neuroscience · 2024Review
- Network pharmacology approach and experimental verification of Dan-Shen Decoction in the treatment of ischemic heart disease.Pharmaceutical biology · 2023Article
- Delayed step-by-step decompression with DSF alleviates skeletal muscle crush injury by inhibiting NLRP3/CASP-1/GSDMD pathway.Cell death discovery · 2023Article
- iPSCs ameliorate hypoxia-induced autophagy and atrophy in C2C12 myotubes via the AMPK/ULK1 pathway.Biological research · 2023Article
- An in vitro evaluation of the antioxidant activities of necroptosis and apoptosis inhibitors: the potential of necrostatin-1 and necrostatin-1i to have radical scavenging activities.Pharmacological reports : PR · 2023Article
- Selenium Deficiency Induces Apoptosis and Necroptosis Through ROS/MAPK Signal in Human Uterine Smooth Muscle Cells.Biological trace element research · 2022Article
- Shenxiong glucose injection inhibits oxidative stress and apoptosis to ameliorate isoproterenol-induced myocardial ischemia in rats and improve the function of HUVECs exposed to CoClFrontiers in pharmacology · 2022Article
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7 authors at 2 institutions in 1 country.
Funding
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Abstract
Necrostatin-1 (Nec-1) is a selective and potent allosteric inhibitor of necroptosis by specifically inhibiting the activity of receptor‑interacting protein (RIP) 1 kinase. The aim of the present study was to determine the effect of Nec‑1 on an anoxia model comprising mouse skeletal C2C12 myotubes. In the present study, a hypoxic mimetic reagent, cobalt chloride (CoCl2), was used to induce hypoxia in C2C12 myotubes. The cytotoxic effects of CoCl2‑induced hypoxia were determined by a Cell Counting kit‑8 assay and flow cytometry. Transmission electron microscopy (TEM) was used to characterize the morphological characteristics of dead cells at the ultrastructural level. To clarify the signaling pathways in CoCl2‑mediated cell death, the expression levels of RIP1, RIP3, extracellular signal‑regulated kinase (ERK)1/2, hypoxia‑inducible factor (HIF)‑1α and B cell lymphoma‑2 adenovirus E1B 19‑kDa interacting protein 3 (BNIP3) were investigated by western blotting. Oxidative stress was determined using 2',7'‑dichlorofluorescin diacetate to measure intracellular reactive oxygen species (ROS) and the fluorescent dye JC‑1 was used to measure mitochondrial membrane potential (Δψm). The results showed that the ratios of apoptotic and necrotic C2C12 cells were increased following CoCl2 treatment, typical necroptotic morphological characteristics were able to observe by TEM, whereas Nec‑1 exhibited a protective effect against CoCl2‑induced oxidative stress. Treatment with Nec‑1 significantly decreased the levels of RIP1, p‑ERK1/2, HIF‑1α, BNIP3 and ROS induced by CoCl2, and promoted C2C12 differentiation. Nec‑1 reversed the CoCl2‑induced decrease in mitochondrial membrane potential. Together, these findings suggested that Nec‑1 protected C2C12 myotubes under conditions of CoCl2-induced hypoxia.
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