ArticleJournal of clinical microbiology2018
Validation of the Pockit Dengue Virus Reagent Set for Rapid Detection of Dengue Virus in Human Serum on a Field-Deployable PCR System.
Article in Journal of clinical microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Prevalence of Chikungunya, Dengue and Zika viruses in blood donors: a systematic literature review and meta-analysis.Blood transfusion = Trasfusione del sangue · 2022Pooled it
- Diagnostic performance of a field-deployable iiPCR platform and viral load dynamics of MPXV Clade IIb in a cohort with high prevalence of HIV.Annals of medicine · 2026Article
- Evaluation of a new NS1 rapid diagnostic test using a single acute-phase serum panel collected during the largest dengue outbreak in Taiwan history in 2015.The Kaohsiung journal of medical sciences · 2022Article
- Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunities.Journal of molecular medicine (Berlin, Germany) · 2020Article
- Dengue Virus and Yellow Fever Virus Detection Using Reverse Transcription-Insulated Isothermal PCR and Comparison with Real-Time RT-PCR.The American journal of tropical medicine and hygiene · 2020Article
- Rapid identification of melioidosis agent by an insulated isothermal PCR on a field-deployable device.PeerJ · 2020Article
- Article
- A review of dengue diagnostics and implications for surveillance and control.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2019Review
- A Direct from Blood/Plasma Reverse Transcription-Polymerase Chain Reaction for Dengue Virus Detection in Point-of-Care Settings.The American journal of tropical medicine and hygiene · 2019Article
- Dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors.Mikrochimica acta · 2019Review
- A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos.PloS one · 2019Article
- An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system.PloS one · 2019Article
- Potential Point-of-Care Testing for Dengue Virus in the Field.Journal of clinical microbiology · 2018Article
- Low frequency of asymptomatic dengue virus-infected donors in blood donor centers during the largest dengue outbreak in Taiwan.PloS one · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dengue virus (DENV) infection, a mosquito-borne disease, is a major public health problem in tropical countries. Point-of-care DENV detection with good sensitivity and specificity enables timely early diagnosis of DENV infection, facilitating effective disease management and control, particularly in regions of low resources. The Pockit dengue virus reagent set (GeneReach Biotech), a reverse transcription insulated isothermal PCR (RT-iiPCR), is available to detect all four serotypes of DENV on the field-deployable Pockit system, which is ready for on-site applications. In this study, analytical and clinical performances of the assay were evaluated. The index assay did not react with 14 non-DENV human viruses, indicating good specificity. Compared to the U.S. CDC DENV-1-4 real-time quantitative RT-PCR (qRT-PCR) assay, testing with serial dilutions of virus-spiked human sera demonstrated that the index assay had detection endpoints that were separately comparable with the 4 serotypes. Excellent reproducibility was observed among repeat tests done by six operators at three sites. In clinical performance, 195 clinical sera collected around Kaohsiung city in 2012 and 21 DENV-4-spiked sera were tested with the RT-iiPCR and qRT-PCR assays in parallel. The 121 (11 DENV-1, 78 DENV-2, 11 DENV-3, and 21 DENV-4) qRT-PCR-positive and 95 qRT-PCR-negative samples were all positive and negative by the RT-iiPCR reagent results, respectively, demonstrating high (100%) interrater agreement (95% confidence interval [CI
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.