ArticleExperimental & applied acarology2018
Identification of spider-mite species and their endosymbionts using multiplex PCR.
Article in Experimental & applied acarology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- The relationship between taxonomic classification and applied entomology: stored product pests as a model group.Journal of insect science (Online) · 2025Review
- Interactions Between EndosymbiontsMicroorganisms · 2025Article
- Exposure to spaceflight enhances the virulence of Purpureocillium lilacinum against Tetranychus cinnabarinus: modulation of the host's enzyme activities and microbiome.Archives of microbiology · 2025Article
- Article
- A Sex-Specific Trade-Off Between Pesticide Resistance and Tolerance to Heat-Induced Sterility inEvolutionary applications · 2024Article
- Environmental Factors and the Symbiont Cardinium Influence the Bacterial Microbiome of Spider Mites Across the Landscape.Microbial ecology · 2023Article
- Population Genomics of Pooled Samples: Unveiling Symbiont Infrapopulation Diversity and Host-Symbiont Coevolution.Life (Basel, Switzerland) · 2023Article
- Identification of Glycycometus malaysiensis (for the first time in Brazil), Blomia tropicalis and Dermatophagoides pteronyssinus through multiplex PCR.Experimental & applied acarology · 2022Article
- A rapid identification method for common astigmatid species based on multiplex polymerase chain reaction.Veterinary research forum : an international quarterly journal · 2022Article
- Molecular characterization of Cardinium, Rickettsia, Spiroplasma and Wolbachia in mite species from citrus orchards.Experimental & applied acarology · 2020Article
- Creating outbred and inbred populations in haplodiploids to measure adaptive responses in the laboratory.Ecology and evolution · 2020Article
- Population-specific effect ofEcology and evolution · 2020Article
- Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions.Heredity · 2020Article
- A New Prevalent Densovirus Discovered inViruses · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Spider mites of the genus Tetranychidae are severe crop pests. In the Mediterranean a few species coexist, but they are difficult to identify based on morphological characters. Additionally, spider mites often harbour several species of endosymbiotic bacteria, which may affect the biology of their hosts. Here, we propose novel, cost-effective, multiplex diagnostic methods allowing a quick identification of spider-mite species as well as of the endosymbionts they carry. First, we developed, and successfully multiplexed in a single PCR, primers to identify Tetranychus urticae, T. evansi and T. ludeni, some of the most common tetranychids found in southwest Europe. Moreover, we demonstrated that this method allows detecting multiple species in a single pool, even at low frequencies (up to 1/100), and can be used on entire mites without DNA extraction. Second, we developed another set of primers to detect spider-mite endosymbionts, namely Wolbachia, Cardinium and Rickettsia in a multiplex PCR, along with a generalist spider-mite primer to control for potential failure of DNA amplification in each PCR. Overall, our method represents a simple, cost-effective and reliable method to identify spider-mite species and their symbionts in natural field populations, as well as to detect contaminations in laboratory rearings. This method may easily be extended to other species.
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