ArticleAfrican journal of laboratory medicine2017
Development of a real-time PCR assay and comparison to CHROMagar
Article in African journal of laboratory medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed, 4 citations in OpenAlex.
- The Global Prevalence of Antibiotic Resistance and Shiga Toxin-ProducingAntibiotics (Basel, Switzerland) · 2025Review
- Evaluation of chromogenic selective agar (CHROMagar STEC) for the direct detection of Shiga toxin-producingJournal of medical microbiology · 2020Article
- Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa.BMC microbiology · 2019Article
- Characterisation of STEC and other diarrheic E. coli isolated on CHROMagar™STEC at a tertiary referral hospital, Cape Town.BMC microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionShiga toxin-producing
objectivesThis project aimed to validate and test an in-house-developed duplex real-time PCR and use it as a reference standard to determine the performance of CHROMagar
methodsThis study evaluated STEC diagnostic technology on African isolates. An in-house-developed duplex real-time PCR assay for detection of
resultsThe real-time PCR limit of detection was 5.3 target copies/μL of broth. The mean melting temperature on melt-curve analysis for detection of
conclusionsThe in-house developed real-time PCR assay is a sensitive and specific option for laboratory detection of STEC as compared to CHROMagar
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.