ArticleScientific reports2018
Isolation and Expansion of Multipotent Progenitors from Human Trabecular Meshwork.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Advances in culture, expansion and mechanistic studies of corneal endothelial cells: a systematic review.Journal of biomedical science · 2019Pooled it
- Bioengineered 3D Human Trabecular Meshwork Models for Outflow Physiology and Glaucoma Research.Bioengineering (Basel, Switzerland) · 2026Review
- A novel glaucoma approach: Stem cell regeneration of the trabecular meshwork.Progress in retinal and eye research · 2022Review
- Significance of serum glucagon-like peptide-1 and matrix Gla protein levels in patients with diabetes and osteoporosis.World journal of clinical cases · 2022Article
- Magnesium and Its Role in Primary Open Angle Glaucoma; A Novel Therapeutic?Frontiers in ophthalmology · 2022Review
- Fundamental Biomaterial Considerations in the Development of a 3D Model Representative of Primary Open Angle Glaucoma.Bioengineering (Basel, Switzerland) · 2021Review
- Replacement of the Trabecular Meshwork Cells-A Way Ahead in IOP Control?Biomolecules · 2021Review
- Article
- Suppression of TGF-β1 signaling by Matrigel via FAK signaling in cultured human trabecular meshwork cells.Scientific reports · 2021Article
- Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye.Pharmaceutics · 2020Review
- Regenerative capacity of the corneal transition zone for endothelial cell therapy.Stem cell research & therapy · 2020Review
- Revisiting Existing Evidence of Corneal Endothelial Progenitors and Their Potential Therapeutic Applications in Corneal Endothelial Dysfunction.Advances in therapy · 2020Review
- α5β1 Integrin Promotes Anchoring and Integration of Transplanted Stem Cells to the Trabecular Meshwork in the Eye for Regeneration.Stem cells and development · 2020Article
- A 3D Model of Human Trabecular Meshwork for the Research Study of Glaucoma.Frontiers in neurology · 2020Review
- Stem Cells from Human Trabecular Meshwork Hold the Potential to Develop into Ocular and Non-Ocular Lineages After Long-Term Storage.Stem cells and development · 2020Article
- Trabecular Meshwork Regeneration - A Potential Treatment for Glaucoma.Current ophthalmology reports · 2019Article
- Trabecular meshwork cells are a valuable resource for cellular therapy of glaucoma.Journal of cellular and molecular medicine · 2019Review
- Human Trabecular Meshwork Progenitors.International journal of medical sciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To expand multi-potent progenitors from human trabecular meshwork (TM), we have created a new optimized method on two-dimensional (2D) followed by three-dimensional (3D) Matrigel in modified embryonic stem cell medium supplemented with 5% fetal bovine serum (MESCM + 5% FBS). The expanded TM cells were small cuboidal cells expressing TM markers such as AQP1, MGP, CHI3L1, and AnkG, embryonic stem cell (ESC) markers such as Oct4, Sox2, Nanog, and ABCG2, and neural crest (NC) markers such as p75NTR, FOXD3, Sox9, Sox10, and MSX1. Although expanded cells lost expression of these markers after passage, the cells regained the markers when Passage 2 cells were seeded on 3D Matrigel through activation of canonical BMP signaling. Such restored progenitors could differentiate into corneal endothelial cells, adipocytes, and chondrocytes but not keratocytes or osteocytes. Therefore, we have concluded that human TM harbors multipotent progenitors that can be effectively isolated and expanded using 2D Matrigel in MESCM + 5% FBS. This unique in vitro model system can be used to understand how TM is altered in glaucoma and whether such TM progenitor cells might one day be used for treating glaucoma or corneal endothelial dysfunction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.