ArticleKidney international2018
A novel assay provides sensitive measurement of physiologically relevant changes in albumin permeability in isolated human and rodent glomeruli.
Article in Kidney international, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Mineralocorticoid receptor antagonism in diabetes reduces albuminuria by preserving the glomerular endothelial glycocalyx.JCI insight · 2023Trial
- Endothelial-erythrocyte glycocalyx exchange enables liquid biopsies of endothelial function.Nature communications · 2026Article
- The glomerular endothelial glycocalyx as a therapeutic target in proteinuric kidney disease.Nature reviews. Nephrology · 2026Review
- Podocyte-directed VEGFC gene therapy prevents increased glomerular permeability and glycocalyx damage in experimental type 1 diabetes.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Article
- Association between glucosamine use and albuminuria in the UK: a cohort and Mendelian randomisation study.BMJ open · 2025Article
- Past and future in vitro and in vivo approaches toward circulating factors and biomarkers in idiopathic nephrotic syndrome.Pediatric nephrology (Berlin, Germany) · 2025Review
- Role of endothelial hyaluronan in peritoneal membrane transport and disease conditions during peritoneal dialysis.Scientific reports · 2024Article
- Endothelial CXCR2 deficiency attenuates renal inflammation and glycocalyx shedding through NF-κB signaling in diabetic kidney disease.Cell communication and signaling : CCS · 2024Article
- Heparanase inhibition as a systemic approach to protect the endothelial glycocalyx and prevent microvascular complications in diabetes.Cardiovascular diabetology · 2024Article
- Physiological Replication of the Human Glomerulus Using a Triple Culture Microphysiological System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The future of intensive care: the study of the microcirculation will help to guide our therapies.Critical care (London, England) · 2023Review
- The microvascular endothelial glycocalyx: An additional piece of the puzzle in veterinary medicine.Veterinary journal (London, England : 1997) · 2022Review
- Visualising the endothelial glycocalyx in dogs.Veterinary journal (London, England : 1997) · 2022Article
- Endothelial glycocalyx is damaged in diabetic cardiomyopathy: angiopoietin 1 restores glycocalyx and improves diastolic function in mice.Diabetologia · 2022Article
- Heparanase as active player in endothelial glycocalyx remodeling.Matrix biology plus · 2022Article
- Selecting the right therapeutic target for kidney disease.Frontiers in pharmacology · 2022Review
- Roles of glomerular endothelial hyaluronan in the development of proteinuria.Physiological reports · 2021Article
- Endothelial Glycocalyx as a Regulator of Fibrotic Processes.International journal of molecular sciences · 2021Review
- Association between urinary salt excretion and albuminuria in Japanese patients with chronic kidney disease: the Fukuoka kidney disease registry study.Clinical and experimental nephrology · 2021Article
- Blocking matrix metalloproteinase-mediated syndecan-4 shedding restores the endothelial glycocalyx and glomerular filtration barrier function in early diabetic kidney disease.Kidney international · 2020Article
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14 authors.
Funding
Abstract
Increased urinary albumin excretion is a key feature of glomerular disease but has limitations as a measure of glomerular permeability. Here we describe a novel assay to measure the apparent albumin permeability of single capillaries in glomeruli, isolated from perfused kidneys cleared of red blood cells. The rate of decline of the albumin concentration within the capillary lumen was quantified using confocal microscopy and used to calculate apparent permeability. The assay was extensively validated and provided robust, reproducible estimates of glomerular albumin permeability. These values were comparable with previous in vivo data, showing this assay could be applied to human as well as rodent glomeruli. To confirm this, we showed that targeted endothelial glycocalyx disruption resulted in increased glomerular albumin permeability in mice. Furthermore, incubation with plasma from patients with post-transplant recurrence of nephrotic syndrome increased albumin permeability in rat glomeruli compared to remission plasma. Finally, in glomeruli isolated from rats with early diabetes there was a significant increase in albumin permeability and loss of endothelial glycocalyx, both of which were ameliorated by angiopoietin-1. Thus, a glomerular permeability assay, producing physiologically relevant values with sufficient sensitivity to measure changes in glomerular permeability and independent of tubular function, was developed and validated. This assay significantly advances the ability to study biology and disease in rodent and human glomeruli.
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