ArticleBioengineered2018
Mycobacterium tuberculosis H37Rv infection regulates alternative splicing in Macrophages.
Article in Bioengineered, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.
- Meta-analysis of active tuberculosis gene expression ascertains host directed drug targets.Frontiers in cellular and infection microbiology · 2022Pooled it
- Review
- Global proteomics reveals pathways of mesenchymal stem cells altered by Mycobacterium tuberculosis.Scientific reports · 2024Article
- The Role of mRNA Alternative Splicing in Macrophages Infected withMolecules (Basel, Switzerland) · 2024Review
- Discovering common pathogenetic processes between tuberculosis and COVID-19 by bioinformatics and system biology approach.Heliyon · 2024Article
- Host factorFrontiers in immunology · 2024Article
- Article
- Analysis of alternative splicing in chicken macrophages infected with avian pathogenicAnimal biotechnology · 2023Article
- From tuberculosis bedside to bench: UBE2B splicing as a potential biomarker and its regulatory mechanism.Signal transduction and targeted therapy · 2023Article
- Sp1 transcription factor represses transcription of phosphatase and tensin homolog to aggravate lung injury in mice with type 2 diabetes mellitus-pulmonary tuberculosis.Bioengineered · 2022Article
- Biological function and molecular mechanism of SRSF3 in cancer and beyond.Oncology letters · 2022Review
- Transcriptome-Based Molecular Networks Uncovered Interplay Between Druggable Genes of CD8Frontiers in medicine · 2021Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe objective of this study was to evaluate the expression of genes encoding SR proteinsand alternative splicing of IL4 and TLR4 in Mycobacterium tuberculosis (M. tb) H37Rv-infected macrophages. MATERIALS AND
methodsTHP-1 cells were induced to differentiate into macrophages with 200 nM PMA, and H37Rv strains were used for macrophage infection. After RNA extraction, qRT-PCR was performed to evaluate the expression of many SR proteins as well as the alternative splicing of IL4 and TLR4.
resultsIL4 and TLR4 play significant roles in host immunity to tuberculosis. The level of IL-4 splice variants in THP-1 cells increased after M. tb H37Rv infection. Three splice variants of TLR4 were detected in M. tb-infected THP-1 cells, when compared with uninfected controls; the expression level of these splicing variants in M. tb-infected THP-1 cell was down-regulated. Since SR proteins are RNA-binding proteins that regulate RNA splicing, the expression of SR proteins was examined, and SRSF2 and SRSF3 were significantly down-regulated. In addition, splicing factors SRp75 and SF3a were also significantly down-regulated post M. tb infection.
conclusionOur findings indicate that alternative splicing may be involved in host gene regulation post M. tb infection of macrophage cells.
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