ArticleNucleic acids research2018
Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- A haloarchaeal VapBC toxin-antitoxin system: regulatory mechanism and role in establishing population heterogeneity to facilitate survival of subpopulations under stress conditions.Applied and environmental microbiology · 2026Article
- A bacterial toxin-antitoxin system as a native defence element against RNA phages.Biology letters · 2025Article
- Discovery of Antimicrobial Agents Based on Structural and Functional Study of theAntibiotics (Basel, Switzerland) · 2024Article
- MazEF Homologs inToxins · 2024Article
- Killing in the name of: T6SS structure and effector diversity.Microbiology (Reading, England) · 2023Review
- Bacterial toxin-antitoxin systems: Novel insights on toxin activation across populations and experimental shortcomings.Current research in microbial sciences · 2023Review
- A secreted effector with a dual role as a toxin and as a transcriptional factor.Nature communications · 2022Article
- Integrative biology of persister cell formation: molecular circuitry, phenotypic diversification and fitness effects.Journal of the Royal Society, Interface · 2022Review
- Type 1 piliated uropathogeniceLife · 2022Article
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- Quantifying heterologous gene expression during ectopic MazF production in Escherichia coli.BMC research notes · 2022Article
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- Article
- A minimal model for gene expression dynamics of bacterial type II toxin-antitoxin systems.Scientific reports · 2021Article
- An Auto-Regulating Type II Toxin-Antitoxin System Modulates Drug Resistance and Virulence inFrontiers in microbiology · 2021Article
- Insights into the global effect on Staphylococcus aureus growth arrest by induction of the endoribonuclease MazF toxin.Nucleic acids research · 2020Article
- Predictive biology: modelling, understanding and harnessing microbial complexity.Nature reviews. Microbiology · 2020Review
- Engineering the Translational Machinery for Biotechnology Applications.Molecular biotechnology · 2020Review
- Trade-offs between gene expression, growth and phenotypic diversity in microbial populations.Current opinion in biotechnology · 2020Review
- Autoregulation of bacterial gene expression: lessons from the MazEF toxin-antitoxin system.Current genetics · 2019Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The MazF toxin sequence-specifically cleaves single-stranded RNA upon various stressful conditions, and it is activated as a part of the mazEF toxin-antitoxin module in Escherichia coli. Although autoregulation of mazEF expression through the MazE antitoxin-dependent transcriptional repression has been biochemically characterized, less is known about post-transcriptional autoregulation, as well as how both of these autoregulatory features affect growth of single cells during conditions that promote MazF production. Here, we demonstrate post-transcriptional autoregulation of mazF expression dynamics by MazF cleaving its own transcript. Single-cell analyses of bacterial populations during ectopic MazF production indicated that two-level autoregulation of mazEF expression influences cell-to-cell growth rate heterogeneity. The increase in growth rate heterogeneity is governed by the MazE antitoxin, and tuned by the MazF-dependent mazF mRNA cleavage. Also, both autoregulatory features grant rapid exit from the stress caused by mazF overexpression. Time-lapse microscopy revealed that MazF-mediated cleavage of mazF mRNA leads to increased temporal variability in length of individual cells during ectopic mazF overexpression, as explained by a stochastic model indicating that mazEF mRNA cleavage underlies temporal fluctuations in MazF levels during stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.