ArticleJournal of experimental botany2018
Integrated regulation triggered by a cryophyte ω-3 desaturase gene confers multiple-stress tolerance in tobacco.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Natural Variation in TaFAD8-D Promoter Enhances Thermotolerance in Wheat Through Fatty Acid and Lipid Remodelling.Plant biotechnology journal · 2026Article
- GhDREB1B-GhFAD3-2 module mediates drought tolerance by regulating linolenic acid biosynthesis in cotton.Science China. Life sciences · 2026Article
- Mechanistic insights into the intrinsic drought resilience ofFrontiers in plant science · 2026Article
- A fijivirus capsid protein hijacks autophagy degrading an ω-3 fatty acid desaturase to suppress jasmonate-mediated antiviral defence.Plant biotechnology journal · 2025Article
- Genome-wide characterization of FAD gene family in Xanthoceras sorbifolium Bunge and germplasm assessment.PloS one · 2025Article
- Proteomic Insights into the Regulatory Mechanisms of the Freezing Response in the Alpine Subnivale PlantInternational journal of molecular sciences · 2024Article
- Rhythmic lipid and gene expression responses to chilling in panicoid grasses.Journal of experimental botany · 2024Article
- Transcriptomic Approach for Investigation ofCurrent issues in molecular biology · 2024Article
- Overexpression of the Purple Perilla (International journal of molecular sciences · 2023Article
- Phytohormones regulate the non-redundant response of ω-3 fatty acid desaturases to low temperatures in Chorispora bungeana.Scientific reports · 2023Article
- Review
- Cell Membrane Features as Potential Breeding Targets to Improve Cold Germination Ability of Seeds.Plants (Basel, Switzerland) · 2022Review
- Fatty acid desaturases (FADs) modulate multiple lipid metabolism pathways to improve plant resistance.Molecular biology reports · 2022Review
- Modulation of GmFAD3 expression alters abiotic stress responses in soybean.Plant molecular biology · 2022Article
- The Absence of Hydrodynamic Stress Promotes Acquisition of Freezing Tolerance and Freeze-Dependent Asexual Reproduction in the Red Alga 'Plants (Basel, Switzerland) · 2021Article
- Physiological and Proteomic Analyses of Different Ecotypes of Reed (Frontiers in plant science · 2021Article
- Decreased R:FR Ratio in Incident White Light Affects the Composition of Barley Leaf Lipidome and Freezing Tolerance in a Temperature-Dependent Manner.International journal of molecular sciences · 2020Article
- Plant Unsaturated Fatty Acids: Biosynthesis and Regulation.Frontiers in plant science · 2020Review
- Plant Unsaturated Fatty Acids: Multiple Roles in Stress Response.Frontiers in plant science · 2020Review
- Root Skewing-Associated Genes Impact the Spaceflight Response ofFrontiers in plant science · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ω-3 fatty acid desaturases (FADs) are thought to contribute to plant stress tolerance mainly through linolenic acid (C18:3)-induced membrane stabilization, but a comprehensive analysis of their roles in stress adaptation is lacking. Here, we isolated a microsomal ω-3 FAD gene (CbFAD3) from a cryophyte (Chorispora bungeana) and elucidated its functions in stress tolerance. CbFAD3, exhibiting a high identity to Arabidopsis AtFAD3, was up-regulated by abiotic stresses. Its functionality was verified by heterogonous expression in yeast. Overexpression of CbFAD3 in tobacco constitutively increased C18:3 in both leaves and roots, which maintained the membrane fluidity, and enhanced plant tolerance to cold, drought, and salt stresses. Notably, the constitutively increased C18:3 induced a sustained activation of plasma membrane Ca2+-ATPase, thereby, changing the stress-induced Ca2+ signaling. The reactive oxygen species (ROS) scavenging system, which was positively correlated with the level of C18:3, was also activated in the transgenic lines. Microarray analysis showed that CbFAD3-overexpressing plants increased the expression of stress-responsive genes, most of which are affected by C18:3, Ca2+, or ROS. Together, CbFAD3 confers tolerance to multiple stresses in tobacco through the C18:3-induced integrated regulation of membrane, Ca2+, ROS, and stress-responsive genes. This is in contrast with previous observations that simply attribute stress tolerance to membrane stabilization.
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