ArticleJournal of experimental botany2018
Direct measurement of intercellular CO2 concentration in a gas-exchange system resolves overestimation using the standard method.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Plant growth regulators enhance growth and photosynthetic performance in endangered Firmiana Kwangsiensis seedlings.BMC plant biology · 2025Article
- Zearalenone and nanozeolite improve Arugula (Eruca sativa) drought tolerance by enhancing photosynthesis and water relations.Scientific reports · 2025Article
- Temperature governs the relative contributions of cuticle and stomata to leaf minimum conductance.The New phytologist · 2025Article
- Physiological Responses ofPlants (Basel, Switzerland) · 2024Article
- GasanalyzeR: advancing reproducible research using a new R package for photosynthesis data workflows.AoB PLANTS · 2024Article
- Accurate photosynthetic parameter estimation at low stomatal conductance: effects of cuticular conductance and instrumental noise.Photosynthesis research · 2024Article
- Photosynthetic responses of Larix kaempferi and Pinus densiflora seedlings are affected by summer extreme heat rather than by extreme precipitation.Scientific reports · 2024Article
- Article
- Rootstock and Crop Load Effects on 'Honeycrisp' Photosynthetic Performance and Carbohydrate Accumulation.Plants (Basel, Switzerland) · 2023Article
- Elevated atmospheric carbon dioxide and plant immunity to fungal pathogens: do the risks outweigh the benefits?The Biochemical journal · 2023Review
- The Interplay of Physiological and Biochemical Response to Short-Term Drought Exposure in Garlic (Plants (Basel, Switzerland) · 2023Article
- An improved theory for calculating leaf gas exchange more precisely accounting for small fluxes.Nature plants · 2021Article
- Constant ratio of CPhotosynthesis research · 2021Article
- A high-performance system of multiple gas-exchange chambers with a laser spectrometer to estimate leaf photosynthesis, stomatal conductance, and mesophyll conductance.Journal of plant research · 2019Article
- Partitioning of mesophyll conductance for COPhotosynthesis research · 2019Article
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3 authors.
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Abstract
Intercellular CO2 concentration of leaves (Ci) is a critical parameter in photosynthesis. Nevertheless, uncertainties in calculating Ci arise as stomata close. Here, by modifying the assimilation chamber of a commercial gas-exchange equipment to directly measure Ci, we demonstrate overestimation of calculated Ci (i.e. Ci(c)) without stimulating stomatal closure. Gas exchange was measured on one side of the leaf while measured Ci (Ci(m)) was acquired simultaneously on the other side of the leaf in hypostomatous passion fruit (Passiflora edulis Sims) and amphistomatous sunflower (Helianthus annuus L.) and common bean (Phaseolus vulgaris L.). The adaxial surface showed comparable Ci(c) and Ci(m) in sunflower, whereas in common bean, where the adaxial surface has a low stomatal density, Ci(c) markedly differed from Ci(m) when the stomata remained open. However, the latter discrepancy disappeared when measuring the leaf flipped upside down so that the gas exchange was measured (i.e. Ci was calculated) on the abaxial side, which has a much higher stomatal density. The passion fruit showed the largest discrepancy on the astomatous side, indicating that the cuticle has a large impact on the calculation. Direct measurement of Ci is recommended as a more accurate estimate than the calculation when stomatal gas transport is restricted. Occurrence of overestimation and prospects for direct measurement are discussed.
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