ArticlePloS one2018
In vitro mouse spermatogenesis with an organ culture method in chemically defined medium.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors.Stem cell research & therapy · 2025Article
- A new humanHuman reproduction open · 2025Article
- Recent Progress of Induced Spermatogenesis In Vitro.International journal of molecular sciences · 2024Review
- The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.Journal of assisted reproduction and genetics · 2024Article
- Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control.The Journal of reproduction and development · 2024Article
- Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis.Stem cell research & therapy · 2023Review
- Human in vitro spermatogenesis as a regenerative therapy - where do we stand?Nature reviews. Urology · 2023Review
- Generation of rat offspring using spermatids produced through in vitro spermatogenesis.Scientific reports · 2023Article
- Ex-Vivo and In-Vivo Expansion of Spermatogonial Stem Cells Using Cell-Seeded Microfluidic Testis Scaffolds and Animal Model.Cell and tissue banking · 2023Article
- Co-Culture of Cryopreserved Healthy Sertoli Cells with Testicular Tissue of Non-Obstructive Azoospermia (NOA) Patients in Culture Media Containing Follicle-Stimulating Hormone (FSH)/Testosterone Has No Advantage in Germ Cell Maturation.Journal of clinical medicine · 2023Article
- Review
- How does retinoic acid (RA) signaling pathway regulate spermatogenesis?Histology and histopathology · 2022Review
- Effect of NMDA receptor agonist and antagonist on spermatogonial stem cells proliferation in 2- and 3- dimensional culture systems.Molecular biology reports · 2022Article
- Article
- Regulation of Cell Types Within Testicular Organoids.Endocrinology · 2021Review
- SCF Improves In Vitro Differentiation of SSCs Through Transcriptionally Up-regulating PRTM1, STRA8, c-KIT, PIWIL2, and OCT4 Genes.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control.Scientific reports · 2021Article
- Chemotherapy induced damage to spermatogonial stem cells in prepubertal mouse in vitro impairs long-term spermatogenesis.Toxicology reports · 2021Article
- Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or AlbuMAX, neither of which were defined chemically. In this study, we formulated a chemically defined medium (CDM) that can induce mouse spermatogenesis under organ culture conditions. It was found that bovine serum albumin (BSA) purified through three different procedures had different effects on spermatogenesis. We also confirmed that retinoic acid (RA) played crucial roles in the onset of spermatogonial differentiation and meiotic initiation. The added lipids exhibited weak promoting effects on spermatogenesis. Lastly, luteinizing hormone (LH), follicle stimulating hormone (FSH), triiodothyronine (T3), and testosterone (T) combined together promoted spermatogenesis until round spermatid production. The CDM, however, was not able to produce elongated spermatids. It was also unable to induce spermatogenesis from the very early neonatal period, before 2 days postpartum, leaving certain factors necessary for spermatogenic induction in mice unidentified. Nonetheless, the present study provided important basic information on testis organ culture and spermatogenesis in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.