ArticleJournal of tissue engineering and regenerative medicine2018
Characterizing human decellularized crystalline lens capsules as a scaffold for corneal endothelial tissue engineering.
Article in Journal of tissue engineering and regenerative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives.International journal of molecular sciences · 2018Pooled it
- Comparison of different decellularization methods for human anterior lens capsules.BMC ophthalmology · 2025Article
- Review
- Advancements in Polymer Biomaterials as Scaffolds for Corneal Endothelium Tissue Engineering.Polymers · 2024Review
- Femtosecond Laser Cutting of Human Crystalline Lens Capsule and Decellularization for Corneal Endothelial Bioengineering.Bioengineering (Basel, Switzerland) · 2024Article
- Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature.Journal of ophthalmology · 2024Review
- An effective method for culturing functional human corneal endothelial cells using a xenogeneic free culture medium.Scientific reports · 2023Article
- The Innovative Biomaterials and Technologies for Developing Corneal Endothelium Tissue Engineering Scaffolds: A Review and Prospect.Bioengineering (Basel, Switzerland) · 2023Review
- Fabrication Strategies for Engineered Thin Membranous Tissues.ACS applied bio materials · 2023Review
- The effect of decellularization protocols on characterizations of thermoresponsive and light-curable corneal extracellular matrix hydrogels.Scientific reports · 2023Article
- Corneal endothelial transplantation from bench to bedside: A review of animal models and their translational value for therapeutic development.Experimental eye research · 2022Review
- Comparative culture of human corneal endothelial cells following treatment with human platelet lysate/fibrin hydrogel versus Y-27632 ROCK inhibitor: in vitro and ex vivo study.International ophthalmology · 2022Article
- Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering.Bioactive materials · 2022Review
- Potential of a novel scaffold composed of human platelet lysate and fibrin for human corneal endothelial cells.Cell and tissue banking · 2022Article
- Ex vivo expansion and characterization of human corneal endothelium for transplantation: a review.Stem cell research & therapy · 2021Review
- Exploring the Mesenchymal Stem Cell Secretome for Corneal Endothelial Proliferation.Stem cells international · 2020Article
- Decellularization methods for developing porcine corneal xenografts and future perspectives.Xenotransplantation · 2019Review
- Poly-ε-lysine based hydrogels as synthetic substrates for the expansion of corneal endothelial cells for transplantation.Journal of materials science. Materials in medicine · 2019Article
- Characterizing human decellularized crystalline lens capsules as a scaffold for corneal endothelial tissue engineering.Journal of tissue engineering and regenerative medicine · 2018Article
- Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells.Stem cells international · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The idea of transplanting a sheet of laboratory-grown corneal endothelium dates back to 1978; however, the ideal scaffold is still lacking. We hypothesized that human crystalline lens capsules (LCs) could qualify as a scaffold and aimed to characterize the properties of this material for endothelial tissue engineering. LCs were isolated from donor eyes, stored at -80 °C, and decellularized with water and trypsin-EDTA. The decellularization was investigated by nuclear staining and counting and the capsule thickness was determined by optical coherence tomography and compared with Descemet's membrane (DM). Transparency was examined by spectrometry, and collagenase degradation was performed to evaluate its resistance to degradation. Cell-scaffold interaction was assessed by measuring focal adhesions surface area on LC and plastic. Finally, primary corneal endothelial cells were grown on LCs to validate the phenotype. Trypsin-EDTA decellularized most effectively, removing 99% of cells. The mean LC thickness was 35.76 ± 0.43 μm, whereas DM measured 25.93 ± 0.26 μm (p < .0001). Light transmission was 90% for both LC and DM. On a collagenase challenge, LC and amniotic membrane were digested after 13 hr, whereas DM was digested after 17 hr. The surface area of focal adhesions for cells grown on coated LCs was at least double that compared with other conditions, whereas tight junctions, ion pumps, and hexagonal morphology were well maintained when endothelial cells were cultured on LCs. In conclusion, LCs demonstrate excellent scaffolding properties for tissue engineering and sustain the cell phenotype and can be considered a suitable substrate for ocular tissue engineering or as a template for future scaffolds.
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