Evidence map›Paper›PMID 29430847›Full record

ArticleChembiochem : a European journal of chemical biology2018

Dual Labeling of the CBP/p300 KIX Domain for

Clifford T Gee, Keith E Arntson, Edward J Koleski, Rachel Lynn Staebell, William C K Pomerantz

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Methods for the Investigation of Protein-Ligands Interactions.Advances in experimental medicine and biology · 2026
    Review
  2. Leveraging relaxation-optimizedNature chemistry · 2025
    Article
  3. Fluorine-Thiol Displacement Stapling on the Disordered αSynlett : accounts and rapid communications in synthetic organic chemistry · 2025
    Article
  4. Review
  5. Article
  6. Garcinolic Acid Distinguishes Between GACKIX Domains and Modulates Interaction Networks.Chembiochem : a European journal of chemical biology · 2023
    Article
  7. RSC chemical biology · 2021
    Review
  8. Article
  9. SAR by (Protein-Observed)Accounts of chemical research · 2019
    Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Clifford T GeeDepartment of Chemistry, University of Minnesota, 207 Pleasant Street, SE, Minneapolis, MN, 55455, USA.
Keith E ArntsonDepartment of Chemistry, University of Minnesota, 207 Pleasant Street, SE, Minneapolis, MN, 55455, USA.
Edward J KoleskiDepartment of Chemistry, University of Minnesota, 207 Pleasant Street, SE, Minneapolis, MN, 55455, USA.
Rachel Lynn StaebellDepartment of Chemistry, University of Minnesota, 207 Pleasant Street, SE, Minneapolis, MN, 55455, USA.
William C K PomerantzDepartment of Chemistry, University of Minnesota, 207 Pleasant Street, SE, Minneapolis, MN, 55455, USA.ORCID 0000-0002-0163-4078
University of Minnesota · US

Funding

Chemistry-Biology Interface Training GrantT32GM008700 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI DISTEFANO, MARK D · 1999 to 2018
$4.0M
NIGMS NIH HHS T32 GM008700
6 · The paper itself

Abstract

Protein-Observed Fluorine NMR (PrOF NMR) spectroscopy is an emerging technique for screening and characterizing small-molecule-protein interactions. The choice of which amino acid to label for PrOF NMR can be critical for analysis. Here we report the first use of a protein containing two different fluoroaromatic amino acids for NMR studies. Using the KIX domain of the CBP/p300 as a model system, we examine ligand binding of several small-molecule compounds elaborated from our previous fragment screen and identify a new ligand binding site distinct from those used by native transcription factors. This site was further supported by computational modeling (FTMap and Schrödinger) and

Indexed as

AnimalsBinding SitesCREB-Binding ProteinCyclic AMP Response Element-Binding ProteinFluorineHumansMiceModels, MolecularNuclear Magnetic Resonance, Biomolecularp300-CBP Transcription FactorsProtein BindingProtein DomainsProtein Interaction MapsRatsSmall Molecule LibrariesCREB-Binding ProteinCyclic AMP Response Element-Binding ProteinFluorinep300-CBP Transcription FactorsSmall Molecule Librariesallosterismbiomolecular NMRfluorineNMR spectroscopyprotein-protein interactions

Identifiers

PMID29430847
PMCPMC6251716
OpenAlexW2802660338

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.