ArticlePlant molecular biology2018
An intronless form of the tobacco extensin gene terminator strongly enhances transient gene expression in plant leaves.
Article in Plant molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Transgenic Tobacco as a Bioreactor for the Production of Bioactive and Triple-Helical Recombinant Type III Collagen.Plants (Basel, Switzerland) · 2026Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- A novel geminivirus-derived 3' flanking sequence of terminator mediates the gene expression enhancement.Plant biotechnology journal · 2025Article
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Incorporation of regulatory DNA elements within a viral vector improves recombinant protein expression in plants.Scientific reports · 2024Article
- High-Level Production of a Recombinant Protein inInternational journal of molecular sciences · 2024Article
- Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella.Plant cell reports · 2024Article
- Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023Review
- Transcriptional and post-transcriptional controls for tuning gene expression in plants.Current opinion in plant biology · 2023Review
- Plant Promoters and Terminators for High-Precision Bioengineering.Biodesign research · 2023Review
- Design of an artificial transcriptional system for production of high levels of recombinant proteins in tobacco (Frontiers in plant science · 2023Article
- Improving Protein Quantity and Quality-The Next Level of Plant Molecular Farming.International journal of molecular sciences · 2022Review
- Identification of a Transferrable Terminator Element That Inhibits Small RNA Production and Improves Transgene Expression Levels.Frontiers in plant science · 2022Article
- Evolutionary conservation of nested MIR159 structural microRNA genes and their promoter characterization inFrontiers in plant science · 2022Article
- The transcription and export complex THO/TREX contributes to transcription termination in plants.PLoS genetics · 2020Article
- Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression.Frontiers in plant science · 2020Review
- Vaccine synergy with virus-like particle and immune complex platforms for delivery of human papillomavirus L2 antigen.Vaccine · 2019Article
- High Level Production of Monoclonal Antibodies Using an Optimized Plant Expression System.Frontiers in bioengineering and biotechnology · 2019Article
- Article
- Chimeric 3' flanking regions strongly enhance gene expression in plants.Plant biotechnology journal · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
key messageWe have found interesting features of a plant gene (extensin) 3' flanking region, including extremely efficient polyadenylation which greatly improves transient expression of transgenes when an intron is removed. Its use will greatly benefit studies of gene expression in plants, research in molecular biology, and applications for recombinant proteins. Plants are a promising platform for the production of recombinant proteins. To express high-value proteins in plants efficiently, the optimization of expression cassettes using appropriate regulatory sequences is critical. Here, we characterize the activity of the tobacco extensin (Ext) gene terminator by transient expression in Nicotiana benthamiana, tobacco, and lettuce. Ext is a member of the hydroxyproline-rich glycoprotein (HRGP) superfamily and constitutes the major protein component of cell walls. The present study demonstrates that the Ext terminator with its native intron removed increased transient gene expression up to 13.5-fold compared to previously established terminators. The enhanced transgene expression was correlated with increased mRNA accumulation and reduced levels of read-through transcripts, which could impair gene expression. Analysis of transcript 3'-ends found that the majority of polyadenylated transcripts were cleaved at a YA dinucleotide downstream from a canonical AAUAAA motif and a UG-rich region, both of which were found to be highly conserved among related extensin terminators. Deletion of either of these regions eliminated most of the activity of the terminator. Additionally, a 45 nt polypurine sequence ~ 175 nt upstream from the polyadenylation sites was found to also be necessary for the enhanced expression. We conclude that the use of Ext terminator has great potential to benefit the production of recombinant proteins in plants.
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