Evidence map›Paper›PMID 29429072›Full record

ArticleFunctional & integrative genomics2018

An ex vivo ruminal ovine model to study the immediate immune response in the context of bacterial lipopolysaccharide.

Fathi Abouhajer, Saeed El-Ashram, Musafiri Karama, Shujian Huang, Jian-Feng Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Functional & integrative genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Frontiers in veterinary science · 2022
    Article
  3. Article
  4. The Neurotropic ParasiteFrontiers in medicine · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 4 countries.

Fathi AbouhajerKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Saeed El-AshramCollege of life Science and Engineering, Foshan University, 18 Jiangwan Street, Foshan, Guangdong, 528231, China. saeed_elashram@yahoo.com.
Musafiri KaramaDepartment of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Shujian HuangCollege of life Science and Engineering, Foshan University, 18 Jiangwan Street, Foshan, Guangdong, 528231, China.
Jian-Feng LiuKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
China Agricultural University · CNFoshan University · CNUniversity of Pretoria · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have set up an ex vivo ovine ruminal model, which can mimic the multicellular process to explore the early steps in Salmonella typhimurium lipopolysaccharide (LPS) stimulation using RNA-seq technology. Ovine ruminal explants were collected for histological and transcriptional analysis and supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. A total of 8 and 523 genes were significantly over-expressed between LPS-treated and control tissues at 6 and 12 h, respectively. However, six and seven hundred and thirteen genes were substantially repressed between the aforementioned tissues, correspondingly. Key genes up-regulated in response to the addition of LPS were tumor necrosis factor (TNF), interlukin (IL)-1 beta(b), IL-6, IL-8, IL-17B, IL-19, MMP-1, MMP-3, and integrin alpha 2 (ITGA8, 9). This study shows for the first time that galectin-1 is up-regulated in an ex vivo ruminal segment model exposed to bacterial lipopolysaccharide following 6 h of incubation. The ruminal segment model has been shown to be a suitable tool to study the bacterial lipopolysaccharide effects on the ovine ruminal tissues prior to in vivo assessment.

Indexed as

Disease Models, AnimalAnimalsGene Expression ProfilingIntegrinsInterleukinsLipopolysaccharidesMatrix MetalloproteinasesRumenSalmonella InfectionsSheepTissue Culture TechniquesTranscriptomeTumor Necrosis Factor-alphaIntegrinsInterleukinsLipopolysaccharidesMatrix MetalloproteinasesTumor Necrosis Factor-alphaGalectin-1LPSOvine ruminal explants

Identifiers

PMID29429072
OpenAlexW2791092709

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.