ReviewApplied and environmental microbiology2018
The Madness of Microbiome: Attempting To Find Consensus "Best Practice" for 16S Microbiome Studies.
Review in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 277 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
277 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- From culture-based to 16S sequencing approaches: oral microbiota changes in aging, frailty, and oral hypofunction - a systematic review.BMC oral health · 2026Pooled it
- Meta-analysis suggests the microbiome responds to Evolve and Resequence experiments in Drosophila melanogaster.BMC microbiology · 2021Pooled it
- The Effect of Exogenous Sex Steroids on the Vaginal Microbiota: A Systematic Review.Frontiers in cellular and infection microbiology · 2021Pooled it
- Phocaeicola vulgatus promote growth rate via tryptophan metabolism pathway mediated gut sIgA production in Taihe Silky fowl.Journal of advanced research · 2026Article
- Multi‑omics insights into uveitis: From mechanisms to precision medicine (Review).International journal of molecular medicine · 2026Review
- Rumen Microbiota, Predicted Metabolic Pathways, and Fermentation Parameters Exhibit Production-System-Specific Associations in Cattle and Goats.Microorganisms · 2026Article
- Strengths and weaknesses in techniques employed to measure the effects of dietary modulation on intestinal microbiota composition and function.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Review
- Intermittent Fasting and the Gut Microbiota: Mechanisms Linking Microbial Remodeling to Metabolic and Immune Regulation.Nutrients · 2026Review
- Comparison of skin microbiota profiles in chronic scratch lesions using tape strip and swab sampling.JAAD international · 2026Article
- Gut Microbiota and Ageing: Mechanisms, Age-Related Diseases, and Therapeutic Perspectives.Healthcare (Basel, Switzerland) · 2026Review
- Deterministic selection and compositional turnover in Parkinson's disease-associated gut dysbiosis.Antonie van Leeuwenhoek · 2026Article
- Mapping potential pathogen profiling in cetacean blow: comparative insights from sequencing technologies.Microbial genomics · 2026Article
- From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR.Molecular ecology resources · 2026Article
- Article
- The gastric ecosystem: assessing resident microbiome vs. transient microorganisms in obesity and following bariatric interventions.NPJ biofilms and microbiomes · 2026Review
- Quantitative evaluation of microbiome sequencing resolution under varying experimental conditions using defined mock communities.Scientific reports · 2026Article
- An Integrated Dataset of Clinical and Microbial Profiles for Fecal Microbiota Transplantation.Scientific data · 2026Article
- The Role of Selected Bacteria in Breast Cancer Initiation and Development.International journal of molecular sciences · 2026Review
- Effects of bacillin 20 on microbial community structure and cross-domain networks in the soybean root-rhizosphere interface.BMC microbiology · 2026Article
- Enhancing DNA recovery in low-biomass snow algae samples: a comparative study of extraction methods and their effect on community composition.Applied and environmental microbiology · 2026Article
217 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The development and continuous improvement of high-throughput sequencing platforms have stimulated interest in the study of complex microbial communities. Currently, the most popular sequencing approach to study microbial community composition and dynamics is targeted 16S rRNA gene metabarcoding. To prepare samples for sequencing, there are a variety of processing steps, each with the potential to introduce bias at the data analysis stage. In this short review, key information from the literature pertaining to each processing step is described, and consequently, general recommendations for future 16S rRNA gene metabarcoding experiments are made.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.