ArticleApplied and environmental microbiology2018
Effect of Laser Irradiation on Cell Function and Its Implications in Raman Spectroscopy.
Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Raman signatures ofFrontiers in microbiology · 2026Article
- Toward smart and in-situ mycotoxin detection in food via vibrational spectroscopy and machine learning.Food chemistry: X · 2025Review
- Comparing Raman and NanoSIMS for heavy water labeling of single cells.Microbiology spectrum · 2025Article
- Laser-stimulated human gingival fibroblasts: alterations in migration, secretome production, and induction of reactive oxygen species.Lasers in medical science · 2025Article
- Light-field tomographic fluorescence lifetime imaging microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Raman-Activated, Interactive Sorting of Isotope-Labeled Bacteria.Sensors (Basel, Switzerland) · 2024Article
- Raman-Activated Cell Ejection for Validating the Reliability of the Raman Fingerprint Database of Foodborne Pathogens.Foods (Basel, Switzerland) · 2024Article
- Raman cell sorting for single-cell research.Frontiers in bioengineering and biotechnology · 2024Review
- Light-field tomographic fluorescence lifetime imaging microscopy.Research square · 2023Article
- Black Silicon: Breaking through the Everlasting Cost vs. Effectivity Trade-Off for SERS Substrates.Materials (Basel, Switzerland) · 2023Article
- Visualization of a Limonene Synthesis Metabolon Inside Living Bacteria by Hyperspectral SRS Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Single-cell Raman-activated sorting and cultivation (scRACS-Culture) for assessing and mining in situ phosphate-solubilizing microbes from nature.ISME communications · 2022Article
- Nanoscale Chemical Imaging of Human Cell Membranes Using Tip-Enhanced Raman Spectroscopy.Angewandte Chemie (International ed. in English) · 2022Article
- Petri-plate, bacteria, and laser optical scattering sensor.Frontiers in cellular and infection microbiology · 2022Review
- One-Cell Metabolic Phenotyping and Sequencing of Soil Microbiome by Raman-Activated Gravity-Driven Encapsulation (RAGE).mSystems · 2021Article
- Effect of high-power Nd:YAG laser on the growth ofJournal of physical therapy science · 2021Article
- Microbial phenomics linking the phenotype to fonction: The potential of Raman spectroscopy.Journal of microbiology (Seoul, Korea) · 2021Review
- Efficacy of single versus multiple exposure by electromagnetic modalities on gram-negative and positive bacterial strains in an in-vitro model.Saudi journal of biological sciences · 2021Article
- Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions.Nature protocols · 2021Article
- Development overview of Raman-activated cell sorting devoted to bacterial detection at single-cell level.Applied microbiology and biotechnology · 2021Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lasers are instrumental in advanced bioimaging and Raman spectroscopy. However, they are also well known for their destructive effects on living organisms, leading to concerns about the adverse effects of laser technologies. To implement Raman spectroscopy for cell analysis and manipulation, such as Raman-activated cell sorting, it is crucial to identify nondestructive conditions for living cells. Here, we evaluated quantitatively the effect of 532-nm laser irradiation on bacterial cell fate and growth at the single-cell level. Using a purpose-built microfluidic platform, we were able to quantify the growth characteristics, i.e., specific growth rates and lag times of individual cells, as well as the survival rate of a population in conjunction with Raman spectroscopy. Representative Gram-negative and Gram-positive species show similar trends in response to a laser irradiation dose. Laser irradiation could compromise the physiological function of cells, and the degree of destruction is both dose and strain dependent, ranging from reduced cell growth to a complete loss of cell metabolic activity and finally to physical disintegration. Gram-positive bacterial cells are more susceptible than Gram-negative bacterial strains to irradiation-induced damage. By directly correlating Raman acquisition with single-cell growth characteristics, we provide evidence of nondestructive characteristics of Raman spectroscopy on individual bacterial cells. However, while strong Raman signals can be obtained without causing cell death, the variety of responses from different strains and from individual cells justifies careful evaluation of Raman acquisition conditions if cell viability is critical.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.