ArticleHistochemistry and cell biology2018
Three-dimensional ultrastructure of osteocytes assessed by focused ion beam-scanning electron microscopy (FIB-SEM).
Article in Histochemistry and cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Review
- Characterization of human trabecular bone across multiple length scales using a correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM.Scientific reports · 2024Article
- Deep learning to overcome Zernike phase-contrast nanoCT artifacts for automated micro-nano porosity segmentation in bone.Journal of synchrotron radiation · 2024Article
- Detailing the influence of PEO-coated biodegradable Mg-based implants on the lacuno-canalicular network in sheep bone: A pilot study.Bioactive materials · 2023Article
- Multiscale Femoral Neck Imaging and Multimodal Trabeculae Quality Characterization in an Osteoporotic Bone Sample.Materials (Basel, Switzerland) · 2022Article
- Correlative volume-imaging using combined array tomography and FIB-SEM tomography with beam deceleration for 3D architecture visualization in tissue.Microscopy (Oxford, England) · 2022Article
- Hierarchical organization of bone in three dimensions: A twist of twists.Journal of structural biology: X · 2022Review
- High-resolution image-based simulation reveals membrane strain concentration on osteocyte processes caused by tethering elements.Biomechanics and modeling in mechanobiology · 2021Article
- Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone.Histochemistry and cell biology · 2021Article
- In focus in HCB.Histochemistry and cell biology · 2020Article
- Osteocytic Osteolysis in PTH-treated Wild-type andThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Review
- Next-generation imaging of the skeletal system and its blood supply.Nature reviews. Rheumatology · 2019Review
- A network of trans-cortical capillaries as mainstay for blood circulation in long bones.Nature metabolism · 2019Article
- In focus in HCB: Hard Tissue Biology.Histochemistry and cell biology · 2018Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study is to demonstrate the application of focused ion beam-scanning electron microscopy, FIB-SEM for revealing the three-dimensional features of osteocytic cytoplasmic processes in metaphyseal (immature) and diaphyseal (mature) trabeculae. Tibiae of eight-week-old male mice were fixed with aldehyde solution, and treated with block staining prior to FIB-SEM observation. While two-dimensional backscattered SEM images showed osteocytes' cytoplasmic processes in a fragmented fashion, three-dimensional reconstructions of FIB-SEM images demonstrated that osteocytes in primary metaphyseal trabeculae extended their cytoplasmic processes randomly, thus maintaining contact with neighboring osteocytes and osteoblasts. In contrast, diaphyseal osteocytes extended thin cytoplasmic processes from their cell bodies, which ran perpendicular to the bone surface. In addition, these osteocytes featured thick processes that branched into thinner, transverse cytoplasmic processes; at some point, however, these transverse processes bend at a right angle to run perpendicular to the bone surface. Osteoblasts also possessed thicker cytoplasmic processes that branched off as thinner processes, which then connected with cytoplasmic processes of neighboring osteocytes. Thus, FIB-SEM is a useful technology for visualizing the three-dimensional structures of osteocytes and their cytoplasmic processes.
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