Evidence map›Paper›PMID 29427073›Full record

ArticleCellular and molecular life sciences : CMLS2018

Dissecting the biochemical architecture and morphological release pathways of the human platelet extracellular vesiculome.

Silvia H De Paoli, Tseday Z Tegegn, Oumsalama K Elhelu, Michael B Strader, Mehulkumar Patel, Lukas L Diduch, Ivan D Tarandovskiy, Yong Wu, Jiwen Zheng, Mikhail V Ovanesov and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Article
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  14. Review
  15. Article
  16. Platelet-Derived Exosomes and Atherothrombosis.Frontiers in cardiovascular medicine · 2022
    Review
  17. Review
  18. Extracellular Vesicles in Blood: Sources, Effects, and Applications.International journal of molecular sciences · 2021
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Silvia H De PaoliLaboratory of Cellular Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Room 4210, Silver Spring, MD, USA.
Tseday Z TegegnLaboratory of Cellular Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Room 4210, Silver Spring, MD, USA.
Oumsalama K ElheluLaboratory of Cellular Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Room 4210, Silver Spring, MD, USA.
Michael B StraderLaboratory of Biochemistry and Vascular Biology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Silver Spring, MD, 20993-0002, USA.
Mehulkumar PatelLaboratory of Cellular Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Room 4210, Silver Spring, MD, USA.
Lukas L DiduchDakota Consulting, Inc., 1110 Bonifant St., Silver Spring, MD, USA.
Ivan D TarandovskiyLaboratory of Cellular Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Room 4210, Silver Spring, MD, USA.
Yong WuDivision of Biology, Chemistry and Materials Science, Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD, USA.
Jiwen ZhengDivision of Biology, Chemistry and Materials Science, Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD, USA.
Mikhail V OvanesovHemostasis Branch, Division of Plasma Protein Therapeutics, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD, USA.
Abdu AlayashLaboratory of Biochemistry and Vascular Biology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Silver Spring, MD, 20993-0002, USA.
Jan SimakLaboratory of Cellular Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, WO Bldg. 52/72, Room 4210, Silver Spring, MD, USA. jan.simak@fda.hhs.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Platelet extracellular vesicles (PEVs) have emerged as potential mediators in intercellular communication. PEVs exhibit several activities with pathophysiological importance and may serve as diagnostic biomarkers. Here, imaging and analytical techniques were employed to unveil morphological pathways of the release, structure, composition, and surface properties of PEVs derived from human platelets (PLTs) activated with the thrombin receptor activating peptide (TRAP). Based on extensive electron microscopy analysis, we propose four morphological pathways for PEVs release from TRAP-activated PLTs: (1) plasma membrane budding, (2) extrusion of multivesicular α-granules and cytoplasmic vacuoles, (3) plasma membrane blistering and (4) "pearling" of PLT pseudopodia. The PLT extracellular vesiculome encompasses ectosomes, exosomes, free mitochondria, mitochondria-containing vesicles, "podiasomes" and PLT "ghosts". Interestingly, a flow cytometry showed a population of TOM20

Indexed as

Blood PlateletsCell-Derived MicroparticlesCell MembraneChemokinesCytokinesExosomesHumansLipidsMembrane Transport ProteinsMicroscopy, ConfocalMicroscopy, Electron, TransmissionMicrotubule-Associated ProteinsMitochondrial Precursor Protein Import Complex ProteinsMitophagyParticle SizePeptide FragmentsChemokinesCytokinesLipidsMAP1LC3A protein, humanMembrane Transport ProteinsMicrotubule-Associated ProteinsMitochondrial Precursor Protein Import Complex ProteinsPeptide FragmentsReceptors, Cell SurfaceSNARE ProteinsThrombinthrombin receptor peptide SFLLRNPTOMM20 protein, humanEctosomeExosomeExtracellular vesiclesMembrane microparticlesMembrane pearlingMitophagyPlateletThrombin generation

Identifiers

PMID29427073
PMCPMC11105464

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.