ArticleScientific reports2018
Label-free Raman hyperspectral imaging analysis localizes the cyanogenic glucoside dhurrin to the cytoplasm in sorghum cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Estimating sorghum leaf dhurrin content using a handheld near infrared instrument.Frontiers in plant science · 2026Article
- A Review of Recent Advances in Chromatographic Quantification Methods for Cyanogenic Glycosides.Molecules (Basel, Switzerland) · 2024Review
- Dhurrin in Sorghum: Biosynthesis, Regulation, Biological Function and Challenges for Animal Production.Plants (Basel, Switzerland) · 2024Review
- Cell Responsiveness to Physical Energies: Paving the Way to Decipher a Morphogenetic Code.International journal of molecular sciences · 2022Review
- Cyanogenesis in theGenes · 2022Article
- Article
- High-Fat Diet Impairs Mouse Median Eminence: A Study by Transmission and Scanning Electron Microscopy Coupled with Raman Spectroscopy.International journal of molecular sciences · 2021Article
- Physical energies to the rescue of damaged tissues.World journal of stem cells · 2019Review
- Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars.Scientific reports · 2019Article
- Raman spectrum spectral imaging revealing the molecular mechanism of Berberine-induced Jurkat cell apoptosis and the receptor-mediated Berberine delivery system.Biomedical optics express · 2019Article
- Dynamic metabolic solutions to the sessile life style of plants.Natural product reports · 2018Review
- Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Localisation of metabolites in sorghum coleoptiles using Raman hyperspectral imaging analysis was compared in wild type plants and mutants that lack cyanogenic glucosides. This novel method allows high spatial resolution in situ localization by detecting functional groups associated with cyanogenic glucosides using vibrational spectroscopy. Raman hyperspectral imaging revealed that dhurrin was found mainly surrounding epidermal, cortical and vascular tissue, with the greatest amount in cortical tissue. Numerous "hotspots" demonstrated dhurrin to be located within both cell walls and cytoplasm adpressed towards the plasmamembrane and not in the vacuole as previously reported. The high concentration of dhurrin in the outer cortical and epidermal cell layers is consistent with its role in defence against herbivory. This demonstrates the ability of Raman hyperspectral imaging to locate cyanogenic glucosides in intact tissues, avoiding possible perturbations and imprecision that may accompany methods that rely on bulk tissue extraction methods, such as protoplast isolation.
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