ArticleScientific reports2018
Reference gene selection for RT-qPCR analysis in Harmonia axyridis, a global invasive lady beetle.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Selection and evaluation of reference genes for RT-qPCR normalization across developmental stages and tissues in Dermestes maculatus (Coleoptera: Dermestidae).Journal of insect science (Online) · 2026Article
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- Stability analysis of reference genes in Maconellicoccus hirsutus across various conditions including dsRNA treatment.Scientific reports · 2025Article
- Selection and evaluation of reference genes for quantitative real-time polymerase chain reaction normalization in Pieris melete (Lepidoptera, Pieridae).Journal of insect science (Online) · 2025Article
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- Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis inInsects · 2024Article
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- Evaluation of reference genes for quantitative expression analysis inFrontiers in physiology · 2024Article
- Identification and Validation of Reference Genes for Expression Analysis Using qRT-PCR inInsects · 2022Article
- Involvement of glucose transporter 4 in ovarian development and reproductive maturation of Harmonia axyridis (Coleoptera: Coccinellidae).Insect science · 2022Article
- Identifying optimal reference genes for gene expression studies in Eurasian spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae).Scientific reports · 2022Article
- Identification and Validation of Reference Genes for Gene Expression Analysis inFrontiers in physiology · 2022Article
- Evaluation of Reference Genes inGenes · 2021Article
- Article
- Selection and Validation of Reference Genes for RT-qPCR Normalization inFrontiers in physiology · 2021Article
- Selection and Validation of Reference Genes For qRT-PCR Analysis ofFrontiers in physiology · 2021Article
- Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis inFrontiers in physiology · 2021Article
- Selection and validation of experimental condition-specific reference genes for qRT-PCR in Metopolophium dirhodum (Walker) (Hemiptera: Aphididae).Scientific reports · 2020Article
Corrections and comments
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Harmonia axyridis is a voracious predator, a biological control agent, and one of the world most invasive insect species. The advent of next-generation sequencing platforms has propelled entomological research into the genomics and post-genomics era. Real-time quantitative PCR (RT-qPCR), a primary tool for gene expression analysis, is a core technique governs the genomic research. The selection of internal reference genes, however, can significantly impact the interpretation of RT-qPCR results. The overall goal of this study is to identify the reference genes in the highly invasive H. axyridis. Our central hypothesis is that the suitable reference genes for RT-qPCR analysis can be selected from housekeeping genes. To test this hypothesis, the stability of nine housekeeping genes, including 18S, 28S, ACTB, ATP1A1, GAPDH, HSP70, HSP90, RP49, and ATP6V1A, were investigated under both biotic (developmental time, tissue and sex), and abiotic (temperature, photoperiod, in vivo RNAi) conditions. Gene expression profiles were analyzed by geNorm, Normfinder, BestKeeper, and the ΔCt method. Our combined results recommend a specific set of reference genes for each experimental condition. With the recent influx of genomic information for H. axyridis, this study lays the foundation for an in-depth omics dissection of biological invasion in this emerging model.
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